陕西人群中IL-8、IL-10单核苷酸基因多态性与结直肠癌的易感性  

Association between IL-8-251A/T and IL-10-1082A/G genetic polymorphisms and susceptibility to colorectal cancer: A case-control study in a population in Shaanxi

作　　者：李红霞[1] 李媛媛[1] 宋政军[1] 和水祥[1] 郭秦乐 

机构地区：[1]西安交通大学医学院第一附属医院消化内科,陕西省西安市710061

出　　处：《世界华人消化杂志》2015年第7期1184-1190,共7页World Chinese Journal of Digestology

摘　　要：目的:探讨陕西人群中白介素-8(interleukin-8,IL-8)-251A/T、IL-10-1082A/G单核苷酸基因多态性(single nucleotide polymorphism,SNP)与结直肠癌的遗传易感性.方法:采用病例对照研究的方法,应用等位特异性聚合酶链式反应(allele specific polymerase chain reaction,AS-PCR),分别检测102例结直肠癌患者和105例健康对照人群中IL-8-251A/T、IL-10-1082A/G的基因分布,运用χ2检验的方法比较2个多态性位点中不同基因型与结直肠癌的易感性.结果:在陕西人群中,结直肠癌组和健康对照组中IL-8-251A/T位点的3种基因型分布差异有统计学意义(χ2=8.278,P=0.016),A等位基因在结直肠癌组中的频率高于对照组(χ2=5.083,P=0.024),携带AA基因型的个体更易患结直肠癌(OR=3.84,95%CI:1.44-10.23);分层分析显示:携带A等位基因年龄<55岁或吸烟的个体,相对于年龄≥55岁或非吸烟者更易罹患结直肠癌(P=0.003).两组间IL-10-1082A/G位点的3种基因型分布差异无统计学意义(χ2=1.808,P=0.405).结论:相同环境条件下,陕西人群中携带IL-8-251A等位基因者增加结直肠癌发生的危险性,尤其是在年龄<55岁及吸烟的个体中,考虑IL-8-251位点的SNP可能是结直肠癌的易感基因;IL-10-1082A/G与结直肠癌的发病无明显相关性.AIM：To explore the association between IL-8-251A/T and IL-10-1082A/G polymorphisms and susceptibility to colorectal cancer（CRC） in the population in Shaanxi,China.METHODS：A case-control study was performed to compare the distribution of genetic frequencies and genotype of IL-8-251A/T and IL-10-1082A/G among colorectal cancer patients（n = 102） and control healthy individuals（n = 105） using allele specific PCR（AS-PCR）.The relationship between IL-8-251A/T and IL-10-1082A/G polymorphisms and susceptibility to colorectal cancer was explored.RESULTS：There was a significant difference in the distribution of genotype of IL-8-251A/T between the healthy control group and CRC group（χ~2 = 8.278,P = 0.016）.The genetic frequency of IL-8-251 A allele in CRC was 0.54（111/204）,which was significantly higher than that of the controls（0.43,91/210）（χ~2 = 5.083;P = 0.024）.The subjects carrying AA genotype were more likely to suffer from CRC（OR = 3.84,95%CI：1.44-10.23）.Stratified analysis suggested that the association between the AA genotype of IL-8-251A/T and the risk of CRC was more profound in subjects less than 55 years old and smokers.However,no difference was discovered in the distribution of genotype of IL-10-1082A/G between the CRC group and healthy control group（χ~2 = 1.808,P =0.405）.CONCLUSION：The IL-8-251 AA genotype may be a risk factor of CRC in the population in Shaanxi,and people with A allele may be more likely to suffer from CRC,especially in those less than 55 years old and smokers.No correlation is found between IL-10-1082A/G polymorphism and the risk of CRC.

关 键 词：结直肠癌 白介素-8-251A/T 白介素-10-1082A/G 基因多态性 

分 类 号：R735.34[医药卫生—肿瘤]