拟南芥GH3.9基因的过表达及其表型分析  

Overexpression and Phenotype Analysis of GH3.9 Gene in Arabidopsis thaliana

作　　者：周苹[1] 唐冬英[1] 郭明[1] 谭振华[1] 赵小英[1] 刘选明[1] 

机构地区：[1]湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室,长沙410082

出　　处：《西北植物学报》2015年第3期454-458,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：湖南省自然科学基金(11JJA002);湖南省生物发育工程及新产品研发协同创新中心(20134486)

摘　　要：为研究GH3.9基因在植物生长发育过程中的作用,利用RT-PCR成功克隆到GH3.9基因,该基因全长为1 750bp。通过构建pEGAD-GH3.9过表达载体转化拟南芥,获得过表达GH3.9基因纯系转基因株系GH3.9ox-3和GH3.9ox-7。对拟南芥野生型(WT)和转基因株系(GH3.9ox-3和GH3.9ox-7)幼苗用不同光强和光质进行处理,结果显示:在蓝光、红光、远红光等不同光照强度下培养,过表达株系幼苗下胚轴的生长均明显受到抑制,且较野生型明显;采用不同光周期处理拟南芥幼苗,过表达幼苗下胚轴的伸长明显低于野生型;对成年植株表型进行观察,发现过表达株系植株矮小、雄蕊变短、果荚短小。研究表明:GH3.9基因参与了拟南芥生长发育调控,过表达GH3.9基因对拟南芥生长有抑制作用。To investigate the role of GH3.9in plant growth and development,we cloned the 1 750 bp fulllength gene with RT-PCR method and obtained GH3.9ox-3and GH3.9ox-9pure lines of Arabidopsis thaliana by constructing pEGAD-GH3.9overexpression vector and transforming A.thaliana.After treating the sowed wild-type and mutant seeds（GH3.9ox-3and GH3.9ox-9）with blue,red and far-red light at different intensity,we found that：the growth of hypocotyls in the mutants was more sensitive to the strong light suppression comparing with wild-type.Moreover,the hypocotyls of mutants appeared to grow slower when treated different forms of white light.The mutants plant sizes and silique sizes appeared smaller.The results indicate that GH3.9gene might play an important role in the growth and development of plants.

关 键 词：拟南芥 GH3.9基因 过表达 表型分析 

分 类 号：Q785[生物学—分子生物学] Q789