大鼠骨髓间充质干细胞增殖及定向分化能力的增龄性变化  

Age-associated proliferation and differentiation changes of rat bone marrow mesenchymal stem cells

作　　者：李海瑞[1] 郑栋[1] 江灿[1] 郭军[1] 张爱东[1] 李自成[1] 

机构地区：[1]暨南大学附属第一医院心血管内科,广东省广州市510630

出　　处：《中国组织工程研究》2015年第1期18-23,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年项目资助(81100078);教育部科学研究重点项目资助(211207);广州珠江科技新星资助计划项目(2012J2200063);广东省科技厅博士启动项目资助(S2011040001392)~~

摘　　要：背景:自体骨髓间充质干细胞移植治疗心血管疾病已经成为当前研究的热点之一,但是患者自体骨髓间充质干细胞的增殖能力及向心肌样细胞方向分化的能力是否随年龄的改变而有所变化尚不清楚。目的:探讨大鼠骨髓间充质干细胞生物学特性的增龄性差异。方法:纯化培养不同年龄组SD大鼠骨髓间充质干细胞,流式细胞仪分析其细胞周期,同时骨髓间充质干细胞与乳鼠心肌细胞共培养,细胞免疫荧光技术分析骨髓间充质干细胞心肌肌钙蛋白T的表达。结果与结论:流式细胞仪分析显示处于G0/G1期骨髓间充质干细胞的百分率随年龄的增加而增加;骨髓间充质干细胞的肌钙蛋白T表达率亦随年龄的增加而减少。结果表明大鼠骨髓间充质干细胞的增殖与定向分化能力随着年龄的增长而下降。BACKGROUND：Autologous bone marrow mesenchymal stem cel transplantation for the treatment of cardiovascular disease has become one of the hotspots, but it is unclear whether the proliferation and directional differentiation of autologous bone marrow mesenchymal stem cels varies changes with age. OBJECTIVE： To explore the proliferation and differentiation changes of rat bone mesenchymal stem cels in different ages. METHODS：The bone marrow mesenchymal stem cels from Sprague-Dawley rats in different age groups were purified and cultured, and then examined by flow cytometry in terms of cel cycle. Meanwhile, neonatal rat cardiomyocytes were co-cultured with bone marrow mesenchymal stem cels. The morphologic changes of bone marrow mesenchymal stem cels and the protein expression of troponin T were detected with immunofluorescence technique. RESULTS AND CONCLUSION： The percentage of bone marrow mesenchymal stem cels in G0/G1 phase was increased with age; while the percentage of expression of troponin T proteins-positive bone marrow mesenchymal stem cels were decreased with age. These findings indicate that the proliferation and differentiation abilities of rat bone marrow mesenchymal stem cels descend with age.

关 键 词：骨髓 间质干细胞 肌钙蛋白T 细胞增殖 干细胞 骨髓干细胞 增殖 骨髓间充质干细胞 定向分化 细胞周期 大鼠 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]