骨髓间充质干细胞移植炎症性肠上皮组织中Wnt/β-catenin信号分子的表达  

The expression of Wnt/beta-catenin signaling molecule in inflammatory bowel diseases treated with bone marrow mesenchymal stem cell transplantation

作　　者：邢艳粉 解绪红[1] 苑召虎[1] 崔业佳 李瑜元[2] 聂玉强[2] 魏亚明[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院输血科、临床分子医学及分子诊断实验室,广东省广州市510180 [2]广州医科大学附属广州市第一人民医院消化科、临床分子医学及分子诊断实验室,广东省广州市510180

出　　处：《中国组织工程研究》2015年第1期49-53,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81070385);广州市重大民生专项基金(201300000100)~~

摘　　要：背景:Wnt/β-catenin信号通路是干细胞调控中最关键的信号通路之一,参与调控细胞的增殖与分化。目的:观察Wnt/β-catenin信号通路中主要信号分子在骨髓间充质干细胞移植修复炎性肠疾病过程中的表达状况。方法:采用三硝基苯磺酸灌肠法制作SD大鼠炎症性肠病模型,并将绿色荧光蛋白标记的骨髓间充质干细胞经尾静脉注入体内,以注射生理盐水为移植正常对照,于移植第14和28天处死大鼠,取其大肠组织,采用q RT-PCR的方法进行检测Wnt/β-catenin信号通路中信号分子的表达情况。结果与结论:q RT-PCR检测结果显示,wnt3a和β-catenin在炎症性肠组织中相对表达量明显增加(P<0.05),而c-myc的表达量没有明显变化(P>0.05)。骨髓间充质干细胞移植修复后,wnt3a、β-catenin和c-myc的表达量都显著下降(P<0.05)。结果表明Wnt/β-catenin信号通路在炎症性肠病以及移植骨髓间充质干细胞后的修复过程中起着重要的作用,协助骨髓间充质干细胞向肠上皮细胞分化,促进肠炎症的好转,并修复炎症区域,恢复肠组织的稳态。BACKGROUND：The Wnt/β-catenin signaling pathway is one of the most important signaling pathways in stem cel regulation, which is involved in regulation of cel proliferation and differentiation. OBJECTIVE：To investigate the expression of Wnt/β-catenin main signaling molecule in inflammatory bowel tissues treated with bone marrow mesenchymal stem cel transplantation. METHODS：2,4,6-Trinitrobenzene sulfonic acid was used for establishing inflammatory bowel diseases rat models. Bone marrow mesenchymal stem cels labeled with green fluorescent protein were transplanted into rat modelsviatail vein. Normal saline was injected as control. The expression of Wnt/β-catenin signaling molecule was detected in the large intestine tissue of inflammatory bowel disease rat models by quantitative RT-PCR at 14 and 28 days after transplantation. RESULTS AND CONCLUSION：Real-time quantitative PCR results showed that the expression of Wnt3a andβ-catenin in the inflammatory bowel tissue increased significantly （P〈 0.05）, while no difference in the expression of c-myc （P〉 0.05）. The expressions of Wnt3a, β-catenin and c-myc in the transplantation group were significantly＆amp;nbsp;lower than those in the control group after transplantation （P〈0.05）. These findings indicate that the Wnt/β-catenin signaling pathway plays important roles in inflammatory bowel disease and repair after bone marrow mesenchymal stem cel transplantation, while this pathway may promote stem cels differentiating into intestinal epithelium, promote recovery from inflammatory bowel disease, repair inflammatory area, and restore intestinal tissue homeostasis.

关 键 词：骨髓 间质干细胞移植 肠疾病 信号传导 干细胞 移植 炎症性肠病 骨髓间充质干细胞 WNT/Β-CATENIN信号通路 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]