人参皂苷对不同强度电离辐射损害人造血干细胞的缓解作用  

Ease effect of ginsenoside on different-intensity ionizing radiation damage to human hematopoietic stem cells

作　　者：黄颖[1] 梁晓燕[1] 李骋进 胡军[1] 周理乾[1] 

机构地区：[1]陕西省人民医院,陕西省西安市710068

出　　处：《中国组织工程研究》2015年第1期124-129,共6页Chinese Journal of Tissue Engineering Research

基　　金：陕西省自然科学基金资助项目(2014JM4154)--人参总皂苷对体外培养的CD34+造血干细胞的辐射保护作用~~

摘　　要：背景:国内外诸多学者和机构都在研究如何缓解辐射损害,寻找最合适的药物,而人参皂苷作为名贵中药人参的主要药理成分,具有明显的抗氧化作用。目的:探讨人参总皂苷对不同强度电离辐射损害人造血干细胞的缓解作用及其作用机制。方法:采集健康人脐血,分离培养得到CD34+造血干细胞,分为未处理组和人参皂苷预处理组,之后分别用1,2,5 Gy的X射线对细胞照射24 h。MTT检测细胞增殖活性,应用流式细胞仪测定各组内的活性氧水平,AV/PI双染检测各组细胞的凋亡率,最后用Western blot和Real-time PCR对各组细胞内caspase-3和Nrf-2水平进行定量分析。结果与结论:治疗剂量的电离辐射可以诱导CD34+细胞活性下降,同时诱导其发生凋亡,而且细胞内的活性氧活性也呈现进行性升高,这种作用与电离辐射的剂量相关,用人参皂苷进行预处理后,上述作用可得到明显缓解。Western blot和PCR检测结果显示人参皂苷Rb3可以有效抑制由电离辐射引起的caspase-3表达活性增强,另外还可以促进Nrf-2在CD34+细胞内的表达。结果表明人参皂苷可以有效抑制电离辐射诱导CD34+造血干细胞的损伤,这种保护作用很可能与其抗凋亡和抑制氧化应激作用相关。BACKGROUND：Many domestic and foreign scholars and institutions are studying how to relieve radiation damage and to find the most suitable drug, while ginsenosides as the main pharmacological ingredient of ginseng show significant antioxidant effect. OBJECTIVE：To investigate the ease effect of ginsenosides on human hematopoietic stem cels under different intensities of ionizing radiations. METHODS： The CD34＋ hematopoietic stem cels were isolated from the healthy cord blood. Then the cels were divided into normal group and ginsenoside-pretreated group, respectively, exposed under 1, 2, 5 Gy of X-ray irradiations for 24 hours. Cel viability was detected in irradiated hematopoietic stem cels by MTT assay. Apoptosis was estimated using the folowing assays： Annexin-V assay, caspase-3 mRNA and protein levels. The generation of reactive oxygen species was evaluated, in the presence or absence of ginsenoside in liquid cultures of CD34＋ human hematopoietic stem cels irradiated with 1-, 2- and 5-Gy X-rays, using a flow cytometry assay. The Nrf-2 mRNA and protein levels were also studied by western blot analysis and RT-PCR, respectively.＆amp;nbsp;RESULTS AND CONCLUSION： Ionizing radiation at the therapeutic dose could decrease the viability of CD34＋ cels and induce the cel apoptosis, and meanwhile, the activity of intracelular reactive oxygen species also showed a progressive increase that was correlated with the dose of ionizing radiation. However, ginsenoside pretreatment could relieve these above-mentioned effects. Ginsenoside inhibited the increase in caspase-3 activity induced by ionizing radiation, and additionaly, enhanced the mRNA and protein expressions of Nrf-2 in CD34＋cels. In conclusion, ginsenoside protects CD34＋ hematopoietic stem cels from radiation effects, which is probably correlated with anti-apoptosis and anti-oxidant roles of ginsenosides.

关 键 词：造血干细胞 人参皂甙类 辐射 电离 干细胞 人参皂苷 电离辐射 活性氧 Nrf-2 陕西省自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]