一氧化氮对裸燕麦耐盐性的增强效应  

Enhanced effects of exogenous nitric oxide on salt tolerance of Avena nuda L. seedlings

作　　者：刘建新[1] 王金成[1] 王瑞娟[1] 贾海燕[1] 

机构地区：[1]陇东学院生命科学与技术学院,甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃庆阳745000

出　　处：《生态学杂志》2015年第4期991-996,共6页Chinese Journal of Ecology

基　　金：甘肃省庆阳市科技计划项目(kz2014-19)资助

摘　　要：采用营养液培养方法,以‘定莜6号’裸燕麦为材料,研究外源一氧化氮供体硝普钠(SNP)对100 mmol·L-1Na Cl胁迫下裸燕麦幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明:5μmol·L-1SNP能明显缓解Na Cl胁迫对幼苗生长的抑制作用,显著提高Na Cl胁迫下裸燕麦叶片超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶活性及谷胱甘肽和抗坏血酸含量,降低丙二醛、过氧化氢、超氧阴离子和游离氨基酸含量及过氧化氢酶活性,提高可溶性糖、可溶性蛋白质和脯氨酸含量及K+/Na+比。分析表明,外源一氧化氮通过提高抗氧化能力和渗透溶质积累以及维持K+、Na+平衡,缓解盐胁迫诱导的氧化伤害和生长抑制,从而提高裸燕麦的耐盐性。A naked oat （Avena nuda L.） cuhivar, ‘ Dingyou No. 6＇ was selected as material to investigate the effects of exogenous NO donor, sodium nitroprusside （SNP）, on the growth, active oxygen metabolism and osmotic substances accumulation in leaves of naked oat seedlings under 100 mmol · L^-1 NaCl using nutrient solution cultivation. The results showed that 5 μmol · L^-1 SNP significantly alleviated the inhibitory effect of salt stress on the growth of naked oat seedlings. Besides, exogenous SNP not only significantly increased the activities of superoxide dismutase, peroxidase, ascorbate peroxidase, but also increased the contents of glutathione, ascorbic acid, soluble sugar, soluble protein and proline as well as the K^＋/Na^＋ ratio. Furthermore, SNP significantly decreased the contents of malondialdehyde, hydrogen peroxide, superoxide anion and free amino acid and catalase （CAT） activity in leaves of naked oat seedlings under NaCl stress. Exogenous NO could alleviate the oxidative damage and growth inhibition of naked oat seedlings under salt stress via improving antioxidant ability and osmotica accumulation as well as keeping balance between K^＋ and Na^＋ under NaCl stress, and consequently improve the salt tolerance of plants.

关 键 词：一氧化氮 盐胁迫 裸燕麦 活性氧代谢 渗透溶质 

分 类 号：Q945[生物学—植物学]