一种赭球虫(Ochromonas sp.)的分离鉴定及食藻特性  

Isolation,identification and grazing characteristics of a protozoan species Ocromonas sp.

作　　者：杨晓景[1] 彭玉辅[1] 黄鑫刘丽 冯悦[1] 夏雪山[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500

出　　处：《生态学杂志》2015年第4期1072-1077,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30960005和31360065)资助

摘　　要：从云南滇池分离到一株能吞食微藻的原生动物,根据形态特征及分子生物学鉴定为一种赭球虫（Ochromonas sp.）。本文研究了该原生动物的食藻范围及不同环境因素对其食藻效果的影响。结果表明：该原生动物对惠氏微囊藻（Microcystis wesenbergii）具有很强的吞食能力,其与惠氏微囊藻（Microcystis wesenbergii）共培养至6 d时,微囊藻去除率达到90%以上;该赭球虫吞食微囊藻效果随其浓度增加而增强,在p H值为4-7、25-35℃条件下具有最佳的食藻效果,但高浓度苯酚对其生长有抑制和杀灭作用;该赭球虫易于培养,繁殖迅速,具有生物防控微囊藻水华的应用潜能。A protozoan with the algophagous capability was isolated from the water samples of Dianchi Lake. Based on the morphological and genetic characteristics,it was identified as Ochromonas sp. The main factors influencing the grazing properties of Ochromonas sp. including algae species,protozoa concentration,culture temperature,time,p H and phenol concentration were investigated. The results showed that Ochromonas sp. had a strong grazing capability on Microcystis wesenbergii. When Ochromonas sp. was co-cultured with M. wesenbergii for 6 days,the removal rate of M. wesenbergii was above 90%. Meanwhile,the grazing effect was enhanced with the increase of the initial concentration of the protozoa,and the best grazing capability was found when the culture p H and temperature were 4-7 and 25-35 ℃,respectively. However,high concentration of phenol could inhibit and kill the Ochromonas sp. The Ochromonas sp. was easy to culture and it reproduced rapidly. Therefore,it could be applied to control the water bloom of M.wesenbergii.

关 键 词：赭球虫 原生动物 微囊藻 叶绿素A 生物控藻 

分 类 号：X52[环境科学与工程—环境工程] X174