宫颈上皮内瘤样病变患者宫颈人乳头瘤病毒感染状况的筛查  

Infection status of cervical human papilloma virus in patients with cervical intraepithelial neoplasia

作　　者：程绍云[1] 华裕忠 牟晓峰 刘存[1] 

机构地区：[1]山东省青岛市第三人民医院检验科,266041 [2]山东省青岛市中心医院检验科

出　　处：《中华医学杂志》2015年第11期857-859,共3页National Medical Journal of China

摘　　要：目的 探讨不同级别女性宫颈上皮内瘤样病变患者人乳头瘤病毒（HPV）宫颈感染状况及不同基因型的分布规律,为临床预防、治疗及HPV疫苗的研发、推广提供相关依据.方法 对象为2011年1月至2013年12月就诊于青岛市第三人民医院和青岛市中心医院的823例不同级别宫颈上皮内瘤样病变患者,利用PCR扩增、导流杂交基因芯片技术对患者宫颈脱落细胞进行23种HPV基因分型检测.结果 823例女性宫颈上皮内瘤变患者HPV感染阳性率为47.51％ （391/823）,从2011至2013年呈逐年增加趋势,差异有统计学意义（P＜0.05）,随着宫颈上皮内瘤样病变程度的增加,低危型-HPV和高危型-HPV感染率也在增加,差异有统计学意义（P＜0.05）.HPV感染以单一基因型和二基因型感染为主,占全部基因型的86.19％（337/391）,多基因型感染为0.51％（2/391）;高危型以HPV16亚型为主,低危型以HPV6亚型为主,感染以高危型为主,占76.21％（298/391）,未检测到39、82亚型.结论 宫颈上皮内瘤变患者HPV感染率高,以高危型为主,应给予积极治疗.Objective To explore the human papilloma virus （HPV） infection and genotypes distribution in female patients with cervical intraepithelial neoplasia so as to provide rationales for preventing and treating HPV and developing HPV vaccine.Methods Polymerase chain reaction （PCR） amplification,flow-through hybridization and gene chip were used to identify 23 HPV genotypes in cervical cells collected from 823 female patients with cervical intraepithelial neoplasia at Third Municipal People＇s Hospital and Central Municipal Hospital during January 2011 and December 2013.Results Among them,HPV infections were detected in 47.51% （391/823）.From 2011 to 2013,there was a trend of increase year by year and the difference was statistically significant （P 〈 0.05）.With the greater degree of cervical intraepithelial neoplasia,LR-HPV and HR-HPV infection rate increased.And there were statistically significant differences （P 〈 0.05）.And single or double genotype infection dominated （86.19%）.Multiple genotype infection accounted for 0.51%.And 76.21% of HPV infections were high-risk genotypes and most of them belonged to HPV16.Otherwise,most low-risk genotypes were HPV6.Besides,HPV39 and HPV82 were not detected.Conclusion HPV infections,particularly high-risk ones,are widespread among female patients with condyloma acuminatum or cervical erosion in Qingdao.Thus active clinical therapy should be provided.

关 键 词：尖锐湿疣 人乳头瘤病毒(HPV) 基因型 

分 类 号：R737.33[医药卫生—肿瘤]