Prevention against diffuse spinal cord astrocytoma: can the Notch pathway be a novel treatment target?  

作　　者：Jian-jun Sun Zhen-yu Wang Ling-song Li Hai-yan Yu Yong-sheng Xu Hai-bo Wu Yi Luo Bin Liu Mei Zheng Jin-long Mao Xiao-hui Lou 

机构地区：[1]Department of Neurosurgery, Peking University Third Hospital [2]Peking University [3]China Stem Cell Research Center, Peking University Health Science Center [4]Clinical Stem Cell Center, Peking University Third Hospital [5]Clinical Laboratory of Tissue & Cell Research Center, Department of Biotech Treatment, Logistics College of Chinese People’s Armed Police Force [6]Department of Neuroradiology, Peking University Third Hospital [7]Department of Neurology, Peking University Third Hospital [8]Neurosurgical Department, Peking Union Medical College Hospital [9]Department of Neurosurgery, Rui’an People’s Hospital, Wenzhou Medical University

出　　处：《Neural Regeneration Research》2015年第2期244-251,共8页中国神经再生研究（英文版）

基　　金：supported by grants from Science Foundation of Ministry of Education of China for the Excellent Youth Scholars,No.200800011035;the National Natural Science Foundation of China,No.81200969/H0912

摘　　要：This study was designed to investigate whether the Notch pathway is involved in the develop-ment of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133＋and CD133？ cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7–11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133＋ cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133？ cell suspension, and Notch-immunopositive expres-sion was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133＋ astro-cytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However,it should be emphasized that the sub-cortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133？ astrocytoma cells. However, these ifndings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treat-ment target for diffuse spinal cord astrocytoma.This study was designed to investigate whether the Notch pathway is involved in the develop-ment of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133＋and CD133？ cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7–11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133＋ cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133？ cell suspension, and Notch-immunopositive expres-sion was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133＋ astro-cytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However,it should be emphasized that the sub-cortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133？ astrocytoma cells. However, these ifndings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treat-ment target for diffuse spinal cord astrocytoma.

关 键 词：nerve regeneration ASTROCYTOMA MICE immunodeficiency （BALB/c） mice Notch NESTIN glial fibrillary acidic protein CD133 spinal cord brain MRI neural regeneration 

分 类 号：R739.4[医药卫生—肿瘤]