克罗恩病患者外周血CD3^+、CD4^+和CD8^+ T细胞变化与炎症标记物的关系  

Relationship between Peripheral Blood CD3^+,CD4^+ and CD8^+ T Cells and Inflammation Markers in Patients with Crohn's Disease

作　　者：袁柏思[1] 金鑫鑫[1] 路又可[1] 刘炯[1] 王少东[1] 王震凯[1] 吴琳[1] 汪芳裕[1] 

机构地区：[1]南京军区南京总医院消化内科,210002

出　　处：《胃肠病学》2015年第3期143-146,共4页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金(81270453)资助

摘　　要：背景:克罗恩病(CD)的发病机制与机体免疫应答异常有关,T细胞参与机体免疫应答过程。目的:研究CD患者外周血CD3+、CD4+、CD8+T细胞变化与炎症相关指标的关系。方法:应用流式细胞仪检测26例CD患者(活动期14例和缓解期12例)和8名健康志愿者(对照组)外周血CD3+、CD4+、CD8+T细胞比例,并分析与炎症相关指标(白细胞计数、血小板计数、ESR、CRP、白蛋白和血红蛋白)的相关性。结果:CD活动期CD3+、CD4+、CD8+T细胞比例均明显高于缓解期和对照组(P<0.05),但缓解期与对照组间差异无统计学意义(P>0.05)。CD活动期ESR和CRP明显高于对照组(P<0.05),血红蛋白和白蛋白水平明显低于对照组(P<0.05),CD缓解期白蛋白水平明显低于对照组(P<0.05),而CD活动期和缓解期白细胞计数、血小板计数与对照组相比差异均无统计学意义(P>0.05)。CD3+、CD4+和CD8+T细胞比例与CRP呈正相关、与血红蛋白呈负相关(P<0.05),CD3+、CD4+T细胞比例与ESR呈正相关(P<0.05);CD3+、CD4+和CD8+T细胞比例与白细胞、血小板计数和白蛋白均无关(P>0.05)。结论:CD患者CD3+、CD4+和CD8+T细胞比例随病情活动而升高,并与CRP呈正相关,与血红蛋白呈负相关。Background： Abnormal immune response is involved in the pathogenesis of Crohn ＇s disease（ CD）,and T lymphocytes are the main players in the immune response. Aims： To investigate the relationship between peripheral blood CD3＋,CD4＋and CD8＋T cells and inflammation-related markers in patients with CD. Methods： Proportions of peripheral blood CD3＋,CD4＋and CD8＋T cells were measured by flow cytometry in 26 CD patients（ including 14 patients in active stage and 12 in remission stage） and 8 healthy volunteers（ control group）,and their correlation with inflammation-related markers（ including white blood cell count,platelet count,ESR,CRP,albumin and hemoglobin）were analyzed. Results： Proportions of CD3＋,CD4＋and CD8＋T cells were significantly increased in patients with active CD than those with remission CD and controls（ P〈0. 05）,however,no significant differences were found between remission CD patients and controls（ P〈0. 05）. ESR and CRP in active CD patients were significantly higher than those in controls（ P〈0. 05）,while albumin and hemoglobin levels were significantly decreased（ P〈0. 05）; albumin in remission CD patients was significantly lower than that in controls（ P〈0. 05）. No significant differences in white blood cell count and platelet count were found between active,remission CD patients and controls（ P〉0. 05）. Proportions of CD3＋,CD4＋and CD8＋T cells were positively correlated with CRP,and negatively correlated with hemoglobin（ P〈0. 05）;CD3＋and CD4＋T cells were positively correlated with ESR（ P〈0. 05）. However,CD3＋,CD4＋and CD8＋T cells were not correlated with white blood cell count,platelet count and albumin level（ P〉0. 05）. Conclusions： Proportions of peripheral blood CD3＋,CD4＋and CD8＋T cells are increased with the increase of disease activity in CD,and are positively correlated with CRP,and negatively correlated with hemoglobin.

关 键 词：CROHN病 T淋巴细胞 生物学标记 

分 类 号：R574.62[医药卫生—消化系统]