漆树酸对人前列腺癌细胞LNCaP细胞周期的影响及机制  

Effects and mechanism of anacardic acid on human prostate cancer LNCaP cell cycle

作　　者：刘宁宁[1] 董晓先[2] 华先良[3] 廖宇宁[2] 黄洪标[2] 

机构地区：[1]广州医科大学附属第二医院广州心血管疾病研究所,广州510260 [2]广州医科大学病理生理学教研室,广州510182 [3]湖北省黄石市中心医院湖北理工学院附属医院肿瘤内科,435000

出　　处：《广东医学》2015年第5期657-659,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81201719);广州医科大学博士启动基金资助项目(编号:2013C43)

摘　　要：目的观察漆树酸对人前列腺癌细胞LNCa P细胞周期的影响并分析其机制。方法人前列腺癌细胞LNCa P经漆树酸处理后,MTS法观察不同时间、不同浓度的漆树酸对细胞增殖率的影响,流式细胞术分析细胞周期变化,Western blot方法观察漆树酸作用后细胞周期相关因子CDK4、CDK6、cyclin D1和P27蛋白水平的表达变化。结果 (1)漆树酸时间和浓度依赖性地抑制细胞增殖;(2)经漆树酸处理后的细胞出现生长抑制,细胞主要阻滞在G0/G1期;(3)漆树酸作用细胞后,CDK4、CDK6、cyclin D1蛋白表达水平低于溶剂对照组,而P27蛋白表达水平高于溶剂对照组。结论漆树酸可抑制LNCa P细胞增殖,其机制可能是与细胞周期相关蛋白P27的上调,CDK4、CDK6、cyclin D1表达下调有关,导致细胞阻滞在G0/G1期。Objective To observe the effects and mechanism on cell cycle of anacardic acid in human prostate cancer LNCaP cells.Methods MTS was used to detect cell proliferation rate of LNCaP cells treated with different con -centraions of anacardic acid at different time spots .The cell cycle analysis was determined by flow cytometry , and the changes of cell cycle related CDK4, CDK6, cyclin D1 and P27 were examined by Western blot.Results （1） MTS： the cell proliferation was significantly inhibited by anacardic acid in time -and dose -dependent manners; （2） Flow cytome-try： after treated by anacardic acid, cells growth were inhibited and mainly blocked at G 0 /G1 phase.（3） Western blot：after treated by anacardic acid , CDK4, CDK6 and cyclin D1 were down -regulated while P27 up -regulated.Conclusion Anacardic acid can inhibit LNCaP cell proliferation , which is mediated by the up -regulation of cell cycle -related pro-tein P27, the down -regulation of CDK4, CDK6 and cyclin D1, and the G0 /G1 phase arrest.

关 键 词：漆树酸 前列腺癌 细胞周期 

分 类 号：R737.250.2[医药卫生—肿瘤]