反复性脑缺血丘脑神经元损害的实验研究  

Experimental study on the neuronal damage in the rat thalamus following repeated cerebral ischemia

作　　者：曹仁存[1] 郑彩梅[2] 欧阳尧[1] 

机构地区：[1]成都军区医总医院神经内科,610083 [2]第三军医大学西南医院神经内科

出　　处：《脑与神经疾病杂志》2002年第4期208-210,共3页Journal of Brain and Nervous Diseases

基　　金："九.五"军队医药卫生科研基金项目(96D0 4 0 )

摘　　要：目的 :研究反复性脑缺血丘脑神经元的病理损害及其机制。方法 :应用45Ca放射自显影及光镜对比研究大鼠单次性和反复性脑缺血及 NMDA受体拮抗剂 MK- 80 1治疗后丘脑钙积聚和神经元损害的病理改变。结果 :反复缺血组丘脑异常钙积聚与神经元损害明显重于单次缺血组 ;MK- 80 1能显著减轻和改善丘脑钙积聚与组织病理损害。结论 :反复非致死性短暂脑缺血导致丘脑腹侧神经元显著累积性损害 ,兴奋性氨基酸及 Ca2 +可能起着重要作用。Objective: To study the neuronal pathological damage and its mechanism of thalamus following repeated cerebral ischemia. Methods: 45 Ca autoradiogrphy and light microscopy were used to determine the calcium acumulation and neuroal pathological changes of thalamus following repeated cerebral ischemia in the rats in comparison with the damage following single cerebral is chemia. The effect of MK-801, a NMDA receptor antagonist was also examined. Result: The abnomal calcium accumulation and neuronal injuries were found to be more severe in the thalamus in the group of repeated cerebral ischemia than those in the single ischemic insult group. MK-801 is effective in reducing or ameliorating the calcium accumulation and histopathological damage in the thalamus. Conclusion: The present results suggested that the repeated nonlethal cerbral ischemia resulted in an intense cumlative damage in the ventral thalamus, the excitatory amino acid and Ca 2+ may paly a major role in it.

关 键 词：反复性脑缺血 丘脑神经元损害 实验研究 ^45Ca放射自显影 兴奋性氨基酸 

分 类 号：R743.31[医药卫生—神经病学与精神病学]