沉默婆罗双树样基因4对前列腺癌LNCaP细胞增殖和凋亡生物学行为的影响  

Knockdown of SALL4 expression regulates cell proliferation and apoptosis in prostate cancer LNCaP cells

作　　者：李金[1] 黄海[1] 赖义明 曾乐祥[2] 曹亿[3] 王淦平[1] 陈贤举 余永晟[1] 陈杰青[4] 张思敏[5] 张一鸣[6] 郭正辉[1] 

机构地区：[1]中山大学孙逸仙纪念医院泌尿外科,广东广州510120 [2]U中山大学孙逸仙纪念医院小儿外科,广东广州510120 [3]中山大学孙逸仙纪念医院急诊外科,广东广州510120 [4]深圳市第二人民医院泌尿外科,广东深圳518035 [5]惠州市中心医院泌尿外科,广东惠州516001 [6]南方医科大学珠江医院泌尿外科,广东广州510282

出　　处：《中国病理生理杂志》2015年第3期435-439,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81272807;No.81472382;No.81101947);广东省自然科学基金资助项目(No.S2011010004546;No.S2012010010780)

摘　　要：目的:使用小干扰RNA(siRNA)抑制人前列腺癌LNCa P细胞中婆罗双树样基因4(SALL4)的表达并对抑制效果进行测定,检测沉默(SALL4)后LNCa P细胞增殖、集落形成及凋亡等生物学行为的变化。方法:培养LNCa P细胞,分为si-SALL4组、阴性对照组与空白对照组。Real-time PCR与Western blotting技术检测SALL4 mRNA与蛋白水平。采用MTS比色法观察各组细胞增殖能力的变化,采用集落形成实验观察各组细胞集落形成能力的变化,流式细胞术研究SALL4对细胞凋亡的影响,利用Western blotting方法检测凋亡相关蛋白Bax和Bcl-2的表达情况。结果:与阴性对照组相比,si-SALL4组SALL4 mRNA和蛋白表达水平、细胞增殖能力、细胞集落形成能力均明显降低(P<0.05),而凋亡细胞明显增加(P<0.05),Bcl-2的表达减弱,而Bax的表达增强。结论:通过siRNA技术沉默(SALL4)表达可抑制前列腺癌LNCa P细胞增殖和集落形成能力,促进细胞凋亡,其促进凋亡作用可能与Bcl-2及Bax表达有关。AIM： To investigate the SALL4 expression, proliferation and apoptosis in the LNCaP cells after transfection of SALL4 siRNA.METHODS： The expression of SALL4 at mRNA and protein levels was detected by real-time PCR and Western blotting.MTS assay, colony formation assay and flow cytometry were used to determine the prolifer-ation, colony formation ability and apoptosis of the LNCaP cells.The effect of SALL4 on the expression of Bax and Bcl-2 was analyzed by Western blotting.RESULTS：Compared with negative control group, the expression of SALL4 at mRNA and protein levels in LNCaP cells was down-regulated by transfection of SALL4 siRNA （ P〈0.05 ） .The proliferation rate and colony formation ability were decreased, while apoptosis rate increased in si-SALL4 group （P〈0.05）.Higher expres-sion of Bax and lower expression of Bcl-2 in si-SALL4 group were observed （ P〈0.05 ） .CONCLUSION：Down-regula-tion of SALL4 by siRNA not only suppresses LNCaP cell proliferation and colony formation, but also inhibits Bcl-2 expres-sion and activates Bax expression to induce apoptosis.

关 键 词：SALL4蛋白 前列腺肿瘤 细胞凋亡 

分 类 号：R730.23[医药卫生—肿瘤]