扰乱昼夜节律对小鼠角膜创伤修复的影响  

Disruption of circadian rhythms inhibits wound healing of corneal epithelium in mice

作　　者：刘鹏[1] 刘俊[1] 薛芸霞[2] 宋方[1] 王寒晴 傅婷[2] 夏潮涌[3] 李志杰[1,2] 

机构地区：[1]暨南大学再生医学教育部重点实验室,广东广州510632 [2]暨南大学医学院眼科研究所,广东广州510632 [3]暨南大学医学院组织学与胚胎学教研室,广东广州510632

出　　处：《中国病理生理杂志》2015年第3期499-504,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30672287;No.30772387;No.81070703)

摘　　要：目的:本研究旨在观察昼夜节律对角膜创伤修复的影响。方法:选用C57BL/6雄性小鼠,在小鼠角膜中央直径为2 mm的圆形区域,用高尔夫样刀机械性去除其上皮层,使用荧光素钠显色创伤面积,动态观察上皮层修复的动力学,并观察白细胞、血小板和分裂细胞的动态变化。结果:全昼(12 h light/12 h light,LL)和全夜(12 h dark/12 h dark,DD)组角膜创伤修复速率小于对照组(12 h light/12 h dark,LD),表现为再上皮化延迟、上皮细胞数量降低、血管扩张直径增大、白细胞和血小板募集的发生延迟,但白细胞和血小板数目显著增多。结论:扰乱昼夜节律延迟炎症反应的发生,但加剧炎症反应;延迟再上皮化过程,最终显著抑制角膜创伤修复过程。AIM： To observe the effect of circadian rhythms on wound healing of mouse corneal epithelium. METHODS：The C57BL/6 male mice were used in the study.A part of corneal epithelium （2 mm in diameter） was struck off by a golf-like knife to form a round wound area.The dynamics of epithelial healing in the wound area were ob-served under microscope with fluorescein staining.In addition, with related antibodies and DAPI, the dynamic changes of the neutrophils, platelets and dividing cells were also investigated.RESULTS：The healing rates in LL group （12 h light/12 h light） and DD group （12 h dark/12 h dark） were obviously slower than that in LD group （12 h light/12 h dark）, mainly showing delayed re-epithelialization, decreased epithelial cells, increased diameter of blood vessel, and delayed re-cruitment of neutrophils and platelets, but more cell number.CONCLUSION：Disruption of circadian rhythms significant-ly inhibits the wound healing of corneal epithelium, mainly through delaying the inflammation and re-epithelialization, but aggravating the inflammatory responses.

关 键 词：角膜 昼夜节律 创伤修复 炎症反应 

分 类 号：R363[医药卫生—病理学] R779.1[医药卫生—基础医学]