系统性红斑狼疮中A20表达特点的研究  

Expression of A20 in patients with systemic lupus erythematosus

作　　者：张帆[1,2] 朱丽花[3] 王旭[4] 陈少华[2] 杨力建[2] 吴秀丽[2] 李萡[2] 周毅[3] 李扬秋[2,4] 

机构地区：[1]广东药学院生命科学与生物制药学院广东省生物技术候选药物研究重点实验室,广东广州510006 [2] 暨南大学 医学院血液病研究所,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632 [3]暨南大学第一附属医院风湿免疫科,广东广州510632 [4]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《中国病理生理杂志》2015年第3期518-523,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.91129720)

摘　　要：目的:了解系统性红斑狼疮(SLE)患者外周血中肿瘤坏死因子α诱导蛋白3(TNFAIP3,也称A20)、核因子κB(NF-κB)、黏膜相关淋巴瘤易位基因1(MALT1)及其转录本1(MALT1V1)的mRNA表达特点。方法:采用real-time PCR法检测21例SLE患者(其中合并硬皮症2例,合并类风湿关节炎1例,合并淋巴瘤1例)及31例健康人外周血单个核细胞(PBMC)中MALT1、A20、NF-κB和MALT1V1 mRNA的表达情况。结果:SLE样本中,A20 mRNA表达水平较健康人明显降低(P<0.01),MALT1和NF-κB也较健康人降低(P<0.01)。在健康人组,A20和NF-κB mRNA的表达水平未呈现明显相关性,MALT1和NF-κB则呈正相关(P<0.05);而在SLE组中,A20和NF-κB mRNA则显示出显著正相关(P<0.05),MALT1和NF-κB则不存在相关性。SLE中MALT1V1表达量显著低于正常人(P<0.05),相关性研究还显示,健康人中A20和MALT1V1存在正相关关系(P<0.01),在SLE中则不存在(P>0.05)。结论:本研究首先提供了MALT1-A20-NF-κB通路在SLE中的表达特点。SLE患者异常低表达A20,这可能与患者低免疫耐受相关,其与NF-κB表达的正相关性情况可能与其它因素的调控有关,有待进一步证实。AIM：To investigate the expression and regulation of A20 in healthy individuals and the patients with systemic lupus erythematosus （SLE）.METHODS：The expression levels of A20, NF-κB, MALT1, and MALT1V1 in peripheral blood mononuclear cells （ PBMC） of the patients with SLE （ including 2 cases with scleroderma, 1 case with rheumatoid arthritis, and 1 case with lymphoma） were analyzed by real-time PCR.RESULTS：A significantly lower A20 expression level was found in the PBMC from SLE group compared with the healthy controls, while the expression levels of MALT1 and NF-κB were also decreased.In addition, no significant correlation between A20 and NF-κB expression levels in healthy group was observed, but a positive correlation was found in SLE group （ P〈0.05） .A significant positive corre-lation between MALT1 and NF-κB expression levels in healthy group （ P〈0.05） was observed, and no significant correla-tion was found in SLE group.The expression level of MALT1V1 in SLE group was significantly lower than that in healthy control group, and there was a positive correlation between A20 and MALT1V1 in healthy volunteers （P〈0.01）, but that did not exist in SLE group.CONCLUSION： The characteristics of the expression pattern of MALT1-A20-NF-κB in the SLE patients were presented.Lower level of A20 expression was found in the SLE patients, in particular with other autoim-mune disease or lymphomas, indicating the lower immune tolerance in SLE.The positive correlation of A20 and NF-κB may relate to positive regulation of MALT1.

关 键 词：系统性红斑狼疮 A20 MALT1 NF-ΚB 

分 类 号：R363.2[医药卫生—病理学] R593.241[医药卫生—基础医学]