载钴纳米管种植体涂层的制备及毒性研究  

Preparation and cytotoxicity evaluation of cobalt doped nanotubular implant coating

作　　者：曹灿 赵领洲 宋艳艳 党永刚 张力 张玉梅 

机构地区：[1]第四军医大学口腔医院修复科.军事口腔医学国家重点实验室,西安710032 [2]第四军医大学口腔医院牙周科.军事口腔医学国家重点实验室,西安710032

出　　处：《实用口腔医学杂志》2015年第2期175-179,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:31200716;81070862)

摘　　要：目的:探索钛种植体表面钴元素的加载方法,评估载钴钛种植体的细胞毒性。方法:纯钛表面通过不同电压阳极氧化处理形成不同管径的二氧化钛纳米管涂层。采用水热处理技术在二氧化钛纳米管涂层内加载钴元素,通过水热处理时间调控钴元素加载量。用CCK-8法检测试件表面对骨髓间充质干细胞的细胞毒性。结果:纯钛试件在不同电压和时间的阳极氧化处理后,表面成功形成不同特征的纳米阵列样形貌。经过水热处理成功加载钴元素于纳米管内部。钴元素的加载明显抑制材料表面细胞的增殖。结论:阳极氧化结合水热处理可以实现载钴二氧化钛纳米管种植体涂层的制备,钴元素的增加量需进一步研究。Objective：To prepare a kind of titanium implant doped with cobalt and to study its cytotoxicity.Methods：The surface of the titanium was anodized to form TiO2 nanotube arrays.Different amount of cobalt was doped by hydrothermal treatment,which was controlled by tuning the hydrothermal treatment duration.The cytotoxicity of the cobalt doped nanotubular implant coating on bone marrow stromal cells （BMSCs）was measured by CCK-8.Results：The nanotubular implant coating with different amount of cobalt was fabricated.The proliferation of BMSCs was inhibited by the nanotubular morphology and cobalt doping.Samples formed by hydro-thermal treatment in 0.1 M cobalt acetate showed significantly cytotoxicity.Conclusion：Hydrothermal treatment of anodized titanium is an effective way for developing novel cobalt doped nanotubular implant coating.The proper dose of cobalt doping needs to be further investigated.

关 键 词：钛种植体 二氧化钛纳米管 钴 细胞毒性 CCK-8 

分 类 号：R783.1[医药卫生—口腔医学]