人骨髓间质干细胞诱导成骨分化蛋白质的鉴定及生物信息学分析  

Protein identification and bioinformatics analysis of osteoblast differentiation from human bone marrow mesenchymal stem cells

作　　者：张爱霞[1] 余伟华[2] 王涛[1] 马保锋[2] 徐彩霞[3] 

机构地区：[1]嘉应学院生命科学学院生物工程系,梅州514015 [2]中山大学中山医学院干细胞与组织工程中心,广州510080 [3]中山大学附属第一医院转化医学中心实验室,广州510080

出　　处：《重庆医科大学学报》2015年第2期275-279,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81000681)

摘　　要：目的:通过蛋白质组学和生物信息学技术建立人骨髓间质干细胞(human bone marrow mesenchymal stem cells,h MSCs)诱导成骨分化蛋白表达谱,为进一步阐明h MSCs的成骨分化机制提供基础资料。方法:收集h MSCs诱导成骨分化的全蛋白,应用双向凝胶电泳(two-dimensional gel electrophoresis,2-DE)技术和图像分析软件进行蛋白质点的识别和检测,选择清晰表达的蛋白质点,应用基质辅助激光解析离子化飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectromety,MALDI-TOF-MS)和生物信息学方法进行蛋白质的鉴定和分析。结果:通过MALDI-TOF-MS和生物信息学鉴定了77种蛋白质;建立了h MSCs诱导成骨分化蛋白质表达谱;利用Gene Ontology对这些蛋白按分子功能和生物学途径进行了归类。结论:成功建立了h MSCs诱导成骨分化蛋白质表达谱;为进一步阐明h MSCs成骨分化的分子机制提供了基础资料,为进一步构建h MSCs诱导成骨分化蛋白质表达数据库奠定了坚实的基础。Objective：To establish protein expressing profile of human bone marrow mesenchymal stem cells（h MSCs）which were induced into osteoblasts through proteomic and bioinformatic techniques and to further elucidate the precise molecular mechanism of h MSCs osteoblast differentiation. Methods：The total protein extracts were obtained with cell lysis buffer from h MSCs which were induced into osteoblasts. Two-dimensional gel electrophoresis（2-DE）and 2-DE gel analysis software were used to recognize and test protein spots.Clearly expressed protein spots were selected and were identified and analyzed by matrix-assisted laser desorption ionization-time of flight mass spectromety（MALDI-TOF-MS） and bioinformatics. Results：Protein expressing maps of h MSCs induced into osteoblasts were set up. Seventy-seven proteins were identified by MALDI-TOF-MS and bioinformatics,which were classified by Gene Ontology according to molecular function and biological process. Conclusion：Protein expressing maps of h MSCs induced into osteoblasts are built,which will provide basal information to illuminate the molecular mechanism of h MSCs differentiation into osteoblasts and to build protein expression database of osteoblast differentiation from h MSCs.

关 键 词：骨髓间质干细胞 蛋白质组学 成骨分化 质谱鉴定 生物信息学 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]