MALAT-1对胃癌细胞系SGC7901侵袭和迁移能力的影响  

The effect of MALAT-1 on the capability of invasion and migration in gastric cancer cell SGC7901

作　　者：陈迪[1] 刘佳铭[2] 王凯[1] 王亚芳[3] 张宏博[1] 

机构地区：[1]第四军医大学西京消化病医院,陕西西安7100322 [2]西安市中心医院消化科,陕西西安710003 [3]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2015年第7期885-888,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81272349)

摘　　要：目的:探讨MALAT-1对胃癌细胞系SGC7901侵袭和迁移能力的影响。方法:用实时定量聚合酶链反应(RT-PCR)的方法在mRNA水平测定MALAT-1在20对胃癌组织及癌旁正常组织的表达,以及在GES和SGC7901细胞中的表达。RNA干扰(RNAi)技术下调胃癌细胞系SGC7901中MALAT-1的表达后,以高内涵细胞运动试验检测其运动能力,Transwell实验检测其侵袭和迁移能力。结果:RT-PCR结果显示MALAT-1在胃癌组织中的表达高于癌旁正常组织;相比GES,MALAT-1在SGC7901中的表达升高。下调MALAT-1在SGC7901中的表达后,高内涵细胞运动试验和Transwell实验结果显示SGC7901的运动、侵袭和迁移能力均明显减弱。结论:MALAT-1能够促进胃癌细胞SGC7901的侵袭和迁移能力。Objective： To investigate the influence of MALAT- 1 on the invasion and migration in human gastric cancer cell line SGC7901. Methods： The mRNA levels of MALAT- 1 in 20 pairs of the gastric cancer tissues and adjacent normal tissues,GES and SGC7901 were determined by real- time PCR（ RT- PCR） assays. The influence of MALAT- 1 on the mobility,invasion and migration of gastric cancer cell line SGC7901 were detected using high content analysis and Transwell assay. Results： The results of RT- PCR showed that MALAT- 1 levels were up- regulated in gastric cancer tissues compared with adjacent normal tissues,and the expression level of MALAT- 1 in SGC7901 was significantly higher than that in GES. High content analysis and Transwell assay showed that the mobile,invasive and migratory ability were decreased after down- regulated the expression of MALAT- 1 in gastric cancer cell line SGC7901. Conclusion： MALAT- 1 could enhance the invasive and migratory ability of gastric cancer cell SGC7901.

关 键 词：胃癌 MALAT-1 迁移 侵袭 

分 类 号：R730[医药卫生—肿瘤] R735.2[医药卫生—临床医学]