FoxM1在胶质瘤对卡氮芥化疗抵抗中的作用  

The role of FoxM1 on BCNU resistance in glioma cells

作　　者：张明杰[1] 范承哲[2] 李少一[1] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110004 [2]首都医科大学附属安贞医院神经内科,北京100029

出　　处：《现代肿瘤医学》2015年第8期1044-1047,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172197;81372484)

摘　　要：目的:研究FoxM1在胶质瘤细胞对卡氮芥化疗耐药中的作用,为胶质瘤化疗提供新的靶点和依据。方法:建立对BCNU耐药的胶质瘤细胞株,采取Western blot法检测FoxM1蛋白表达水平的变化;通过RNA干扰技术下调FoxM1表达水平,CCK-8法检测胶质瘤细胞对卡氮芥的敏感性;检测DNA损伤修复基因XRCC1蛋白表达水平。结果:卡氮芥耐药的U251胶质瘤细胞中FoxM1与XRCC1的蛋白表达水平明显升高;下调FoxM1表达水平后,恢复了耐药细胞株对卡氮芥的敏感性;下调FoxM1蛋白表达水平后,XRCC1蛋白表达水平随之下降,与胶质瘤细胞对卡氮芥的敏感程度相关。结论:FoxM1在胶质瘤细胞对卡氮芥耐药过程中发挥重要作用;在这一过程中,DNA损伤修复机制参与了FoxM1介导的胶质瘤细胞对卡氮芥的耐药过程。Objective：To study the role of FoxM1 on BCNU resistance in glioma cells, provides a new target and basis for glioma chemotherapy. Methods： BCNU - resistant glioma cell line was established, the protein expression level of FoxM1 was detected by Western blot. The FoxM1 expression level was reduced by RNA interference, the sen- sitivity of glioma cells to BCNU was detected by CCK - 8 assay. The protein expression level of XRCC1 was detected by Western blot. Results：The expression levels of FoxM1 and XRCC1 were significantly increased in BCNU -resist- ant U251 cells. The sensitivity to BCNU in BCNU - resistant U251 cells was restored by reduce the expression of FoxM1. The protein expression level of XRCC1 was reduced by shRNA - FoxM1 ,associated with the sensitivity of gli- oma cells to BCNU. Conclusion：FoxM1 play an important role in the process of BCNU resistance in glioma cells, DNA damage repair mechanisms is involved in the BCNU resistance mediated by FoxM1 in glioma cells.

关 键 词：FOXM1 卡氮芥 化疗抵抗 

分 类 号：R730[医药卫生—肿瘤] R739.41[医药卫生—临床医学]