外源性p53基因导入对乳腺癌细胞系MCF-7中MDM2表达的影响  

Effect of wild-type p53 gene introduction on the expression of MDM2 in breast cancer cell MCF-7

作　　者：聂明辉 戚林 李志生 王巍[2] 

机构地区：[1]营口市中心医院普通外科,辽宁营口115000 [2]辽宁医学院附属第一医院普外科,辽宁锦州121000

出　　处：《现代肿瘤医学》2015年第8期1048-1050,共3页Journal of Modern Oncology

基　　金：辽宁省高等学校杰出青年学者成长计划(编号:LJQ2011091)

摘　　要：目的:探讨野生型p53导入对乳腺癌细胞系MCF-7生存的影响,及对MDM2表达的影响。方法:人腺病毒p53注射液处理人乳腺癌细胞系MCF-7,将野生型p53基因导入细胞中,用MTT和流式细胞仪研究两组MCF-7细胞的增殖凋亡情况,Western blot检测MDM2蛋白表达情况。结果:外源性野生型p53基因导入组增殖能力低于对照组,凋亡率明显高于对照组,且外源性野生型p53基因导入组MDM2表达明显高于对照组。结论:野生型p53导入可以诱导人乳腺癌细胞系MCF-7凋亡增加,且可以上调MDM2的表达,这可能与外源性p53增加后其负反馈泛素化降解酶也增加有关。Objective：To investigate the effect of wild -type p53 gene introduction in breast cancer cell MCF -7, and to explore the relation of MDM2 expression with wild - type p53 treatment. Methods： Breast cancer cell MCF - 7 was treated with recombinant human adenovirus -p53. The proliferation of MCF -7 was accessed by MTY, the apopto- sis of MCF -7 was investigated by FAS. The expression of MDM2 was detected by Western blot. Results ：The recom- binant human adenovirns - p53 down - regulated the proliferation of breast cancer MCF - 7 accompanied with high apoptosis. We found the up - regulation of MDM2 in the group of recombinant human adenovirus - p53. Conclusion： The recombinant human adenovirus - p53 can induce the apoptosis on the breast cancer MCF - 2,it up - regulated the expression of MDM2. MDM2 expression can be activated by the raise of exogenous wild -type of p53 ,because MDM2 is the major E3 ubiquitin ligase involved in p53 degradation and is critical for regulating p53 homeostasis.

关 键 词：乳腺癌 野生型P53 MDM2 

分 类 号：R737.9[医药卫生—肿瘤]