非小细胞肺癌组织中PEDF、VEGFR-2的表达  

The Expression of PEDF and VEGFR-2 in Patients with Non-small Cell Lung Cancer

作　　者：王世君[1] 黄莉[1] 谢婷婷[1] 夏曙华[1] 

机构地区：[1]贵阳医学院附院临检科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第3期234-236,共3页Journal of Guiyang Medical College

基　　金：高等学校特色专业建设点[教高函(2010)15];贵州省高等学校教改重点项目[黔教高发(2010)208];贵阳医学院科研基金(k2006-37)

摘　　要：目的:检测非小细胞肺癌(NSCLC)组织标本中色素上皮衍生因子(PEDF)、血管内皮生长因子受体(VEGFR-2)的表达,探讨PEDF在NSCLC血管新生中的作用及其与VEGFR-2之间关系。方法:应用免疫组织化学法检测28例NSCLC患者癌组织和远离癌组织的对照组织中PEDF和VEGFR-2的表达;通过标记CD34计数微血管密度(MVD),比较癌组织中PEDF和VEGFR-2阴阳性表达时血管新生情况。结果:癌组织中PEDF和VEGFR-2表达阳性率分别为71.43%、64.29%,与对照组织不表达相比,差异有统计学意义(P<0.05);PEDF和VEGFR-2的共表达率在癌组织和对照组织中分别为39.29%、0%,差异有统计学意义(P<0.01);癌组织MVD(29.30±3.80)低于对照组织(98.86±18.87),差异有统计学意义(P<0.05);癌组织中PEDF阳性表达的MVD显著低于阴性表达的MVD,VEGFR-2阳性表达的MVD显著高于其阴性表达的MVD,差异均有统计学意义(P<0.05)。结论:NSCLC细胞PEDF和VEGFR-2阳性表达百分率高;PEDF阳性表达和VEGFR-2的阴性表达可能与组织MVD有关。Objective： To investigate the expression of pigment epithelium derived factor（ PEDF）and vascular endothelial growth factor receptor（ VEGFR-2） in patients with non-small cell lung cancer（ NSCLC）,the function of PEDF in angiogenesis of non-small cell lung cancer and the relationship with VEGFR-2. Methods： The expressions of PEDF and VEGFR-2 were evaluated by immunohistochemical test in tumor tissues of 28 NSCLC patients and natural tissues. Comparing angiogenesis conditions of positive and negative expressions of PEDF and VEGFR-2 in cancer tissue by marking microvessel density（ MVD） of CD34. Results： The positive rates of PEDF and VEGFR-2 were 71. 43% and64. 29% respectively in tumor tissues,PEDF and VEGFR-2 didn＇t express in control group,there was statistical significance between the cancer group and control group（ P〈0. 05）; the synchronous expression rate of PEDF and VEGFR-2 in cancer group was markedly higher than that in natural tissue（ 39. 29% and 0%）,differences were statistically significant（ P〈0. 01）; MVD（ 29. 30 ± 3. 80） in cancer group was lower than that of natural tissue group（ 98. 86 ± 18. 87）,there was statistical significance（ P〈0. 05）; MVD in cancer tissue which positively expressed PEDF was remarkably lower than that of cancer tissue which negatively expressed PEDF,MVD in cancer tissue which positively expressed VEGFR-2 was remarkably higher than that of cancer tissue which negatively expressed VEGFR-2（ P〈0. 05）. Conclusions： The positive rate of PEDF and VEGFR-2 were high in NSCLC tissue,and there is a correlation of PEDF positive expression and VEGFR-2 negative expression with tissue MVD.

关 键 词：癌 非小细胞肺 色素上皮衍生因子 血管内皮生长因子受体-2 新生血管化 病理性 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]