关于两种全自动生化分析仪校准周期的对比研究  

The Contrastive Analysis of the Calibrating Period between Two Kinds of Fully Automatic Biochemical Analyzers

作　　者：王春雨[1] 李宇周[2] 张桂丽[3] 彭宗生[1] 刘品静 

机构地区：[1]秦皇岛市北戴河医院检验科,河北秦皇岛066100 [2]秦皇岛市卫生学校生物生化组,河北秦皇岛066001 [3]中国煤矿工人北戴河疗养院,河北秦皇岛066100

出　　处：《现代生物医学进展》2015年第7期1328-1331,共4页Progress in Modern Biomedicine

摘　　要：目的:对日立H7180、奥林帕斯Au640全自动生化分析仪校准周期的对比分析。方法:相同条件下,两生化分析仪进行定标校准后,每隔2小时测定高、中、低3种浓度的质控血清各一次直到24 h,后每间隔24 h测定一次,共测定30 d,检测项目均重复测定4次取平均值。从校准完成后开始计时到累积变异系数(CV)大于1/6 CLIA'88允许误差时终止,确定该项目的校准周期。通过绘制校准周期图获得两种全自动生化分析仪不同检测项目的校准周期,并对结果进行对比分析。结果:以碱性磷酸酶(ALP)为例绘制校准周期图,进行对比分析。各检测项目在日立H7180中,低水平质控CV最先达到校准要求,高水平质控CV最后达到校准要求;而在奥林帕斯Au640中,中水平质控CV最先达到校准要求,高水平质控CV最后达到校准要求。结论:日立H7180和奥林帕斯Au640全自动生化分析仪,其校准周期基本一致,个别项目存在差异。Objective： The contrastive analysis of the calibrating period between the fully automatic biochemical analyzers Hitachi H7180 and Olympus Au640 was carried out in this experiment. Methods： Under the same condition, after taking the regular calibration and adjustment for the two biochemical analyzers, test the quality control sera of the high, middle and low concentration levels separately every two hours till 24 hours later, then take the test once every 24 hours, with the total amount of time for 30 days. All the test items will be tested repeatedly four times to gain the average data. The calibrating period of this item starts from the fulfillment of the calibration till the cumulative coefficient of variation （CV） goes beyond 1 / 6 CLIA＇88 permissible error. The calibrating period of the different test items from the two biochemical analyzers was obtained by drawing calibrating periodogram, and contrastive analysis of the results was carried out in this experiment. Results： The calibrating periodograms of alkaline phosphatase （ALP） were drawn and contrasted. The re- suits revealed that when the experiment of every testing item was carried out in Hitachi H7180, the calibrating request was fulfilled at first by the low level quality control CV, and at last by the high level quality control CV; while in Olympus Au640, the calibrating request was fulfilled at first by the middle level control CV, and at last by high level quality control CV. Conclusions： Basically, the two fully au- tomatic biochemical analyzers Hitachi H7180 and Olympus Au640 have the same calibrating period, with variation in some individual items.

关 键 词：生物化学 仪器与设备 校准周期 日立H7180 奥林帕斯Au640 

分 类 号：R446.112[医药卫生—诊断学]