Twist1调控FoxM1在上皮性卵巢癌中的表达  

Twist1 Regulates the Expression of FoxM1 in Epithelial Ovarian Cancer

作　　者：杨丽娜[1,2] 赵静[1] 吴娟[1] 李郁[3] 杨红[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710032 [2]中国兵器工业卫生研究所,陕西西安710065 [3]第四军医大学基础部细胞生物学教研室,陕西西安710032

出　　处：《现代生物医学进展》2015年第8期1452-1456,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81272202)

摘　　要：目的:探讨Twist1调控转录因子Fox M1在上皮性卵巢癌中的表达。方法:采用免疫组织化学SP法检测Fox M1、Twist1在上皮性卵巢癌组织中表达情况,斯皮尔曼秩相关分析其在上皮性卵巢癌组织中的表达相关性;双荧光素酶报告系统检测Twist1对Fox M1的调控作用;Real-time quantitative RT-PCR和蛋白免疫印迹技术验证Twist1对Fox M1的调控作用。结果:Twist1、Fox M1在上皮性卵巢癌组织中的阳性表达率分别是71.4%(40/56)、78.6%(44/56),明显高于正常卵巢组织,差异有统计学意义(P<0.05);且其在上皮性卵巢癌中的表达显著相关(r=0.896,P<0.01);Twist1可以结合并激活Fox M1启动子(P<0.05);上调Twist1表达可以激活Fox M1在卵巢癌细胞中的表达(P<0.05),而干扰Twist1后可以下调Fox M1的表达(P<0.05)。结论:Twist1参与调控增殖相关转录因子Fox M1在卵巢癌中的表达,可能是一个潜在的治疗靶点。Objective： This study aims to explore that Twist1 regulates the expression of transcription factor Fox M1 in human epithelial ovarian cancer. Methods： Immunohistochemical staining（SP） was used to evaluate the expression levels of Fox M1 and Twist1 in human ovarian cancer, Spearman＇s rho was calculated to analyze the correlation between Fox M1 expression and Twist1 expression.Dual-luciferase reporter assay system was employed to test the effect of Twist1 on the expression of Fox M1. Real-time quantitative RT-PCR and Western-blot were exerted to validate regulatory effect of Twist1 on Fox M1. Results： The positive expression rates of Twist1 and Fox M1 in epithelial ovarian cancer were 71.4%（40/56）and 78.6%（44/56）, respectively, which were remarkably higher than those in normal ovarian tissues. The difference was statistically significant（P〈0.01）. Fox M1 had a significant positive correlation with the expression of Twist1 in these epithelial ovarian cancer samples（r=0.896, P〈0.01）.Twist1 was involved in activation of the Fox M1promoter（P〈0.05）. Up regulation of Twist1 can activate expression of Fox M1 in epithelial ovarian cancer（P〈0.05）, and the expression of Fox M1 was decreased significantly when the expression of Twist1 was downregulated（P〈0.05）. Conclusions： Twist1 may be involved in regulation of expression of proliferation-associated transcription factor Fox M1 in human ovarian carcinoma and may serve as a promising therapeutic target for ovarian cancer.

关 键 词：TWIST1 FOXM1 上皮性卵巢癌 表达调控 

分 类 号：R737.31[医药卫生—肿瘤]