大鼠偏头痛模型的制作改进及评判指标选择  

Improvement of Migraine Model in Rats and its Evaluation Indexes

作　　者：姜维[1] 韦华梅[1] 赵美[1] 郭舜[1] 刘新友[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038

出　　处：《现代生物医学进展》2015年第9期1623-1627,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30873440);陕西省中医药管理局资助项目(13-ZY039)

摘　　要：目的：对现有硝酸甘油型大鼠偏头痛模型不均一稳定缺点予以改进,探究更为合理、有效模型建立方法和特征指标。方法：采用雄性SD大鼠,分别使用硝酸甘油无水乙醇注射液和硝酸甘油混悬液,按照12 mg/kg、10 mg/kg、8 mg/kg剂量造模并观察记录其行为学变化。用ELISA法检测模型血清和脑组织5-HT变化,确定较为合理、可靠且均一的改良硝酸甘油偏头痛模型。结果：成功复制大鼠体偏头痛模型。使用硝酸甘油混悬液,皮下注射用量为10 mg/kg时模型效果较均一,造模后20~60 min之间有显著的行为学变化,出现抖身现象,并以倦怠结束。结论：与现有的偏头痛模型比较,最显著的改进之处在于使用硝酸甘油混悬液,延长了模型标志性行为动作的持续时间,以特定时间段内出现明确的行为变化作为观察指标,避免了剂型剂量和个体差异带来的模型效果差异,从而提高了模型的均一性。Objective： To inquiry more reasonable, effective migraine model construction method and characteristic indexes,through improving the disadvantage of inhomogeneity and instability of migraine model rats induced by nitroglycerin. Methods： The migraine model of rats was established by injecting subcutaneously nitroglycerin anhydrous ethanol solution or nitroglycerin suspension with dose of 12 mg/kg, 10 mg/kg, 8 mg/kg, respectively. The behavior changes of rats was observed and recorded. Change of 5-HT in brain and serum of model rats were detected by ELISA. Results： The homogeneous migraine model of rats with nitroglycerin suspension was successfully constructed. The results showed there was significant behavior change after subcutaneous injection of nitroglycerin suspension with dosage of 10 mg/kg between 20 and 60 min. The behavior of shaking the body was detected in model rats and ended in burnout. Conclusion： Compared with existing migraine model, the migraine model constructed with nitroglycerin suspension remarkably prolonged the duration of iconic behavioral changes, moreover, avoided the disadvantages from dosage form dosage and individual difference on model, then greatly improved the uniformity of migraine model.

关 键 词：偏头痛模型 硝酸甘油 行为变化 5-HT 

分 类 号：Q95-3[生物学—动物学] R747.2[医药卫生—神经病学与精神病学]