增强微胶囊在血液中稳定性的研究  

Improvement of Stability of Microcapsules in the Blood

作　　者：陈立[1,2] 张英[1] 王雨[1,2] 李珅[1,2] 李娜[1,2] 于炜婷[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医学材料工程组,辽宁大连116023 [2]中国科学院大学,北京100039

出　　处：《现代生物医学进展》2015年第10期1812-1815,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(210762072;81171550;81101757);中国科学院战略性先导科技专项(XDA01030303)

摘　　要：目的:通过改进成膜工艺,减少血液中HCO3-对海藻酸钠-ε-聚赖氨酸(ε-AP)微胶囊稳定性的影响,从而增强ε-AP微胶囊在血液中的稳定性,使其能够应用于生物人工肝血浆/血液灌注。方法:改进成膜工艺:在成膜液中添加10 mmol/L的Na HCO3并增加ε-聚赖氨酸反应浓度来制备ε-AP微胶囊,用膨胀度表征其机械强度,荧光标记的牛血清白蛋白(FITC-BSA)检测其通透性能,并以微胶囊的膨胀度变化作为衡量指标,考察微胶囊在Na HCO3溶液和血清中的稳定性。结果:改进成膜工艺制备的ε-AP微胶囊与常规条件制备的微胶囊具有接近的机械强度和通透性;改进成膜工艺能够增强的ε-AP微胶囊在Na HCO3溶液和血清中的稳定性;常规条件制备的ε-AP微胶囊在血清中膨胀度增加了约560%,而改进成膜工艺制备的微胶囊膨胀度仅增加160%,其稳定性显著增强。结论:改进成膜工艺能够增强ε-AP微胶囊在血液中的稳定性。Objective： To reduce the impact of HCO3-on the stability of alginate-ε-poly（L-lysine） microcapsules（ε-AP） and improve the stability of ε-AP microcapsules in the blood by adjusting preparation condition for potential application of microcapsule-based bioartificial livers. Methods： ε-AP microcapsules were obtained by adjusting preparation condition. Specifically, 10mmol/L Na HCO3 was added to the microcapsule-forming solution and the concentration of ε-poly（L-lysine） was increased. The mechanical stability of microcapsules was measured by swelling degree and the permeability of microcapsules was evaluated by FITC-labeled bovine serum albumin（BSA）. Then, the stability of microcapsules in Na HCO3 solution and in the blood was studied and measured by the change of swelling degree. Results： Microcapsules were prepared with their mechanical stability and permeability similar to those obtained under conventional preparation condition. The stability of ε-AP microcapsules in the Na HCO3 solution and in the blood was improved by adjusting preparation condition. The swelling degree of conventionally prepared microcapsules in the blood was increased by 560%, while the swelling degree of microcapsules obtained by adjusting preparation condition was only increased by160%. The stability of microcapsules was significantly improved. Conclusions： The stability of ε-AP microcapsules was greatly improved by adjusting preparation condition.

关 键 词：微胶囊 稳定性 生物人工肝 

分 类 号：R318[医药卫生—生物医学工程] Q813[医药卫生—基础医学]