不同低氧时间对SD大鼠颏舌肌肌纤维类型的影响  

Effect of Different Hypoxia Time on the Genioglossus Fiber Types of SD Rats

作　　者：秦文 江丽斌 刘文佳 邵金陵 郭涛 

机构地区：[1]第四军医大学口腔医院正畸科军事口腔医学国家重点实验室,陕西西安710032 [2]第四军医大学口腔医院组织工程研究中心军事口腔医学国家重点实验室,陕西西安710032

出　　处：《现代生物医学进展》2015年第11期2005-2009,2013,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81100058)

摘　　要：目的:观察不同低氧时间大鼠颏舌肌肌纤维类型的变化。方法:建立低氧模型,血气分析证实模型成功建立。在低氧不同时间点分别取低氧组和正常组雄性SD大鼠颏舌肌进行HE染色,肌球蛋白ATP酶组织化学染色和RT-PCR检测肌纤维类型的变化。结果:血气分析结果证实低氧组氧分压与血氧饱和度较对照组发生明显下降(P<0.05)。低氧1周组、2周组、3周组、4周组较正常组氧分压下降至55.04±2.31 mm Hg,52.69±1.51 mm Hg,49.80±1.39 mm Hg,50.11±3.02 mm Hg(P<0.05);血氧饱和度下降至77.51±1.81%,70.13±2.90%,74.20±1.95%,74.97±2.36%(P<0.05)。HE染色和肌球蛋白ATP酶组织化学染色法显示低氧2、3、4周组Ⅱ型肌纤维所占比例较相应正常组依次升高:45.92±1.8%,57.44±2.1%,56.89±2.6%,在第三周时达到顶峰(P<0.05)。RT-PCR结果也同样验证了这一规律。结论:随着低氧活动的进行,颏舌肌肌纤维类型发生有规律的转化,从而影响着肌肉的功能。Military Medical University; Objective： To observe the genioglossus fiber type changes in rats of different hypoxia time. Methods： Establishing the hypobaric hypoxia model, the model was confirmedby blood gas analysis.At different time, the genioglossus was tested by HE staining,myosin ATP enzyme staining and RT-PCR was detected the changes in muscularfiber types. Results： The blood gas analysis showed that both partial pressure of oxygen（PO） and oxygen saturation（OS） in the experimental group（hypobaric hypoxia）was significantly decreased than that of the control group. In hypoxia 1 week, 2 weeks, 3 weeks, 4 weeks groups, the PO was decreased to 55.04 ±2.31 mm Hg, 52.69±1.51 mm Hg, 49.80±1.39 mm Hg, 50.11±3.02 mm Hg（P0.05）; blood oxygen saturation was decreased to77.51±1.81%,70.13 ±2.90%,74.20 ±1.95%, 74.97 ±2.36%（P0.05）. HE staining and myosin ATP enzyme histochemical staining showed that, in hypoxia 2,3,4-week group, compared with normal group, type Ⅱ fibers was increased by45.92±1.8%, 57.44±2.1%, 56.89±2.6% with the peak in the third week. RT-PCR results also verified this rule. Conclusions： The genioglossus fiber types changed regularly along with thehypoxia time which affected the function of muscle.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 低氧 颏舌肌 ATP酶组织化学染色 肌球蛋白重链 

分 类 号：Q95-3[生物学—动物学] R78[医药卫生—口腔医学]