小干扰RNA靶向沉默β-catenin基因对胃癌AGS细胞人端粒酶逆转录酶的影响  

Silencing β-catenin Expression by si RNA on Human Telomerase Reverse Transcriptase of Gastric Cancer AGS cells

作　　者：李三党 景化忠[1] 韩晓鹏[2] 苏琳[2] 刘宏斌[2] 

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730000 [2]兰州军区兰州总医院,甘肃兰州730050

出　　处：《现代生物医学进展》2015年第11期2014-2017,共4页Progress in Modern Biomedicine

基　　金：国家科技部;财政部科技惠民计划项目(2012GS620101);甘肃省科技厅科技重大专项资助项目(2010GS04390)

摘　　要：目的:研究小干扰RNA(short interfering RNA,si RNA)沉默β-catenin基因对胃癌AGS细胞人端粒酶逆转录酶(human telomerase reverse transcriptase,h TERT)的影响。方法:用Lipofectamine 2000将si RNA-β-catenin(实验组)及si RNA-control(阴性对照组)序列转入细胞AGS中,同时设置正常AGS组(空白对照组),运用细胞计数法检测三组细胞增殖能力的变化,同时使用western blot检测三组细胞β-catenin、h TERT的表达。结果:转染72 h后,si RNA-β-catenin组细胞增值能力显著低于si RNA-control组和正常AGS组(P<0.05),si RNA-β-catenin组细胞β-catenin、h TERT表达显著低于si RNA-control组和正常AGS组(P<0.05)。结论:AGS细胞中Wnt/β-catenin信号通路可能参与调控h TERT基因转录。Objective： To research the influence of β-catenin silencing by short interfering RNA（si RNA） on human telomerase reverse transcriptase（h TERT） of AGS cells, and explore its mechanism and relationships. Methods： si RNA-β-catenin（experimental group） and si RNA-control（negative control group） sequences were transfected into AGS cells by Lipofectamine 2000, and set the normal AGS cells group（blank group） at the same time. The three groups of cells proliferation ability were detected by counting method, while the expression of h TERT and β-catenin were determined by Western blot. Results： 72 h after transfection, the proliferation ability of si RNA-β-catenin group were significantly lower than the si RNA-control group and normal AGS group（P 0.05）, and the expression ofβ-catenin and h TERT were significantly lower than si RNA-control group and normal group（P0.05）. Conclusion： Wnt/β-catenin signaling pathway may be involved in the regulation of h TERT gene transcription.

关 键 词：胃癌 Β-CATENIN 人端粒酶逆转录酶 

分 类 号：Q75[生物学—分子生物学] R735.2[医药卫生—肿瘤]