间变性淋巴瘤激酶基因在上皮性卵巢癌上的初步研究  

Preliminary study on anaplastic lymphoma kinase gene in epithelial ovarian cancer

作　　者：印永祥[1] 杨清海[2] 赵华[1] 赵敏[3] 

机构地区：[1]南京医科大学附属无锡妇幼保健院病理科,江苏无锡214002 [2]福州迈新生物技术开发有限公司 [3]南京医科大学附属无锡妇幼保健院妇科

出　　处：《中国妇幼保健》2015年第11期1763-1765,共3页Maternal and Child Health Care of China

基　　金：国家自然青年基金〔81100437〕;无锡市卫生局面上指导性科研项目〔MD201309〕

摘　　要：目的:探讨间变性淋巴瘤激酶(ALK)基因在卵巢癌组织中的表达。方法:选取61例卵巢上皮性癌组织和10例良性卵巢上皮性肿瘤组织、10例正常卵巢组织,应用免疫组织化学方法检测ALK蛋白,ALK阳性者用FISH进行进一步的确认。结果:卵巢良性上皮性肿瘤组织及正常卵巢组织不表达ALK蛋白,ALK基因在卵巢癌组织中的表达阳性率为1.64%。在61例卵巢上皮性癌中,只发现1例年龄超过50岁、临床Ⅰ期和无腹膜转移的ALK蛋白阳性,经FISH确认为EML4-ALK基因阳性。结论:ALK基因可能在卵巢癌的发生发展过程中发挥着协同作用,提示EML4-ALK有可能成为卵巢癌的一种亚型,作为新的靶向治疗指标。Objective： To explore the expression of anaplastic lymphoma kinase（ ALK） gene in epithelial ovarian cancer. Methods： Immunohistochemical method was used to detect ALK proteins expressions in 61 cases with epithelial ovarian cancer,10 cases with benign epithelial ovarian tumor,and 10 cases with normal ovary; the cases with positive ALK were further determined by FISH. Results： ALK protein did not express in benign epithelial ovarian tumor and normal ovary,the positive expression rate of ALK gene in epithelial ovarian cancer was 1. 64%. Among 61 cases with epithelial ovarian cancer,only one case was more than 50 years old; ALK protein expressed positively in the cases of clinical stage Ⅰ and without peritoneal metastasis,then they were determined as positive EML4- ALK gene definitely by FISH. Conclusion： ALK gene may have synergistic effect in oncogenesis and development of ovarian cancer,which indicates that EML4- ALK may become to be a subtype of ovarian cancer,which can be used as a new index for targeted therapy.

关 键 词：上皮性卵巢癌 ALK EML4-ALK FISH 

分 类 号：R737.31[医药卫生—肿瘤]