水牛卵母细胞成熟过程中激活蛋白激酶C对受精后原核形成的影响  

Effect of the activation of protein kinase C on the pronucleus formation of buffalo oocytes after fertilization during the maturation process of buffalo oocytes

作　　者：朱云干[1] 徐椿慧[1] 卞桂华[1,2] 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225000 [2]广西大学动物繁殖研究所,南宁534000

出　　处：《黑龙江畜牧兽医》2015年第4期88-89,95,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金："863"国家高技术研究发展计划项目(2011AA100607);转基因生物新品种培育科技重大专项项目(2010ZX08007-003)

摘　　要：为了研究成熟过程中激活蛋白激酶C(PKC)对水牛卵母细胞受精后原核形成的影响,试验采用水牛卵丘-卵母细胞复合体在不同成熟培养液(即添加PVA、FCS、PMA和PVA的基础成熟液或添加4α-PDD和PVA的基础成熟液)中成熟24 h后,将水牛卵母细胞随机分为5组进行体外受精。结果表明:20小时时检查原核的形成,PMA成熟培养处理的卵母细胞受精后的原核形成率为(66.7±2.3)%,与PVA和FCS阴性对照组[分别为(51.4±3.6)%、(46.8±5.1)%]相比差异不显著(P>0.05),但激活PKC后受精形成原核的卵母细胞数比添加4α-PDD的对照组高且差异显著(P<0.05)。说明在水牛卵母细胞成熟过程中激活PKC有利于提高两原核的形成率。To study the effect of the activation of protein kinase C（ PKC） on the pronucleus formation of buffalo oocytes after fertilization during the maturation process of buffalo oocytes,buffalo oocytes were randomly divided into four groups for in vitro fertilization after buffalo cumulus-oocyte complexes were matured in different maturation mediums including the additions of polyvinyl alcohol（ PVA）,fetal calf serum（ FCS）,phorbol- 12- myristate- 13- acetate（ PMA） with 4- α- phorbol- 12,13- didecanoate（ 4α- PDD）,and PVA with PMA in the basal maturation mediums for 24 h. The results showed that the pronuclearr formation of buffalo oocytes was examined after 20 h. The pronuclear formation rates of the fertilized buffalo oocytes were（ 66. 7 ± 2. 3） %,（ 51. 4 ± 3. 6） %,and（ 46. 8 ± 5. 1） % in the group treated with PMA,PVA and FCS negative control groups,respectively; there were no significant differences（ P〉0. 05） among the groups. However,the number of oocytes fertilized to form pronucleus after the activation of PKC was higher than（ P〈0. 05） that of the control group treated with 4α- PDD. The results indicate that the activation of PKC can improve the formation rate of two pronucleuses during the maturation process of buffalo oocytes.

关 键 词：水牛 卵母细胞 体外成熟 分裂 蛋白激酶C(PKC)激活剂 信号转导 

分 类 号：S823.83[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]