脂多糖诱导炎症对大鼠氧诱导视网膜病变的影响  

Effects of LPS-induced inflammation on oxygen-induced retinopathy in rats

作　　者：李晓琴[1] 张自峰[1] 王雨生[1] 高翔[1] 杨湘敏[1] 徐文芹[1] 

机构地区：[1]第四军医大学西京医院眼科、全军眼科研究所,陕西省西安市710032

出　　处：《眼科新进展》2015年第4期301-304,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81271014;81470655);德国洪堡基金会(Alexander von Humboldt Foundation)仪器设备捐赠基金资助(to YS Wang,V-8151/02085)~~

摘　　要：目的观察不同剂量脂多糖(lipopolysaccharide,LPS)诱导炎症对大鼠氧诱导视网膜病变(oxygen-induced retinopathy,OIR)的影响,以探讨炎症在早产儿视网膜病变(retinopathy of prematurity,ROP)中的作用。方法将新生SD大鼠随机分组,实验组依据LPS注射剂量的不同分为LPS-50、LPS-100和LPS-500组,并分别设立正常对照组(N组)和氧诱导对照组(OIR组)。N组幼鼠喂养于空气中,实验组和OIR组幼鼠饲养于氧气体积分数为80%/21%(24 h交替一次)的氧箱中至出生后14 d(P14),随后移至空气中继续饲养。实验组幼鼠行OIR处理过程中,于P7行LPS腹腔注射,LPS-50、LPS-100和LPS-500组注射剂量分别为50μg·kg-1、100μg·kg-1和500μg·kg-1。检测各组幼鼠在P7(注射前)、P8、P11和P14等不同时间点的体质量变化;并分别于P14和P18制作全视网膜铺片,通过GS-isolectin B4染色观察各组视网膜血管化及病理性新生血管情况。结果在各检测时间点,各实验组和OIR组幼鼠的体质量均低于N组(P<0.05)。P7时,各实验组和OIR组体质量无差别(P>0.05);至P8、P11和P14时,LPS-500组幼鼠体质量明显低于OIR组和其他实验组(P<0.05)。P14时,N组幼鼠的浅层视网膜血管已覆盖整个视网膜;而OIR组和实验组幼鼠的视网膜均存在无血管区,且LPS-500组无血管区面积最大(27.32%±3.58%,P<0.01)。P18时,N组视网膜血管网层次结构清晰;OIR组和实验组幼鼠的视网膜均出现病理性新生血管,且LPS-500组新生血管累及视网膜的范围最广(累及钟点数为6.83±1.72,P<0.01)。结论 LPS诱导炎症可加重大鼠OIR,且在一定范围内呈剂量依赖性,提示炎症可能参与ROP病理过程。Objective To investigate the role of inflammation in retinopathy of prematurity（ ROP） by assessing the effects of different doses of lipopolysaccharide（ LPS） on oxygen-induced retinopathy（ OIR） in rats. Methods Newborn Sprague Dawley rats were randomly divided into treatment group,OIR and normal control groups,and treatment group was subdivided into LPS-50,LPS-100 and LPS-500 subgroups according to doses of LPS injected. Rats of treatment and OIR groups were exposed to alternating 24-hour cycles of hyperoxia（ 80% O2） and normoxia（ 21% O2） for 14 days,while rats of normal control group were maintained in room air.Inflammation in pups of treatment groups was induced by intraperitoneal injection of LPS at postnatal day 7（ P7）,and the doses of LPS for LPS-50,LPS-100,and LPS-500 groups were 50μg·kg- 1,100 μg·kg- 1 and 500 μg·kg- 1,respectively. Body mass of all pups were monitored at P7（ pre-injection）,P8,P11 and P14. Wholemounted and GS-isolectin B4 stainning retinas were analyzed for severity of avascular area and retinal neovascularization at P14 and P18.Results Compared with normal control group,the body mass of pups from treatment and OIR groups were decreased at all time points tested（ P〈0. 05）. At P8,P11 and P14,the body mass of rats in LPS-500 group were significantly lower than those of OIR and other LPS-treated rats（ P〈0. 05）. At P14,the retinal vessels obliterated in the rats of LPS-treated and OIR groups,with the largest retinal avascular area in LPS-500 group（ 27. 32% ± 3. 58%,P 〈0. 01）. At P18,LPS-treated rats and OIR rats displayed overgrowth of abnormal retinal vessels,and the severity of pathologic neovascularization was increased evidently after LPS（ 500 μg·kg- 1） injected（ 6. 83 ± 1. 72,P 〈0. 01）. Conclusion LPS-induced inflammation can aggravate OIR in rats in a dose-dependent manner,which indicates that inflammation might participate in the pathology process of ROP.

关 键 词：炎症 氧诱导视网膜病变 视网膜新生血管 早产儿视网膜病变 

分 类 号：R774.1[医药卫生—眼科]