龙胆泻肝汤对大鼠实验性自身免疫性葡萄膜炎的治疗作用  

Treatment efficacy of Longdan Xiegan Tang on rats with experimental autoimmune uveitis

作　　者：唐凯[1,2] 郭大东[3] 张莲[1] 司俊康 杜宇翔[1] 毕宏生[3,2] 

机构地区：[1]山东中医药大学,山东省济南市250002 [2]山东中医药大学附属眼科医院,山东省济南市250002 [3]山东中医药大学眼科研究所,山东省济南市250002

出　　处：《眼科新进展》2015年第4期305-309,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助编号:81373826);山东省中医药科技发展计划项目(编号:2013ZDZK-083);山东省医药卫生科技发展计划项目(编号:2013WS0251)~~

摘　　要：目的探讨龙胆泻肝汤对大鼠实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)的治疗作用。方法 Lewis大鼠随机分为对照组6只、EAU组18只和LXT组18只,光感受器间维生素A结合蛋白免疫EAU组和LXT组大鼠。观察大鼠生理特征及眼部炎症变化,出现葡萄膜炎症时给予LXT组大鼠龙胆泻肝汤灌胃;免疫后12 d比较各组大鼠眼组织病理学差异;免疫后每隔4 d检测房水蛋白及血清中白蛋白、球蛋白变化。结果成功制备了EAU大鼠模型;免疫后5 d大鼠开始出现葡萄膜炎症状,12 d炎症最重。EAU大鼠肛温在1 d、7 d、10 d和12 d高于对照组(P=0.020、0.000、0.015、0.001);免疫后12 d,EAU组房水蛋白浓度为(36.03±3.23)g·L-1,LXT组为(24.67±2.60)g·L-1,差异有显著统计学意义(P=0.000);组织病理学显示EAU组视网膜结构破坏程度明显高于LXT组(P.000)。EAU组免疫后4 d、8 d及12 d白蛋白均低=0于对照组(P=0.045、0.000、0.033),LXT组4 d和8 d白蛋白低于正常(P=0.045、0.005),但12 d时恢复至正常;12 d时EAU组球蛋白明显高于正常(P=0.047),LXT组未见明显异常。结论龙胆泻肝汤能有效减轻EAU大鼠前房炎症,减少炎症细胞浸润,保护眼部组织结构,增强机体抗氧化能力,调节免疫状态,对EAU发挥治疗作用。Objective To study the treatment efficacy of Longdan Xiegan Tang on rats with experimental autoimmune uveitis（ EAU）. Methods Lewis rats were randomly divided into control group（ 6 rats）,EAU group（ 18 rats） and LXT group（ 18 rats）. Rats in EAU and LXT groups were immunized with interphotoreceptor retinoid-binding protein,and then the changes of physiological features were recorded during the observational duration. Further,rats in LXT group were treated with Longdan Xiegan Tang when inflammation symptom was observed in eyes. The changes of histopathology were compared on day 12 post immunization,and aqueous humor protein,albumin and globulin in serum were detected every 4 days. Results The model of uveitis with burning-heat in the liver and gallbladder type has successfully been constructed. Uveitis inflammation was observed on day 5 and the severity peaked on day12 post immunization. The rectal temperature of EAU rats was higher than those of normal ones on day 1,day 7,day 10 and day 12 post immunization（ P = 0. 020,0. 000,0. 015,0.001）. On day 12,the concentrations of aqueous humor protein were（ 36. 03 ± 3. 23） g·L- 1in EAU group and（ 24. 67 ± 2. 60） g·L- 1 in LXT group, respectively,and there was statistically significant differences（ P = 0. 000）. Meanwhile,the morphologic changes of organizational structure of retina also showed significant differences between EAU group and LXT group on day 12（ P = 0. 000）. Moreover,the levels of albumin in EAU group on day 4,day 8 and day 12 post immunization were lower than that in the control group（ P = 0. 045,0. 000,0.033）,the levels of albumin in LXT group on day 4 and day 8 post immunization were lower than that in the control group（ P = 0. 045,0. 005）,and returned to the normal at day 12; The level of globulin in EAU group on day 12 post immunization was higher than that in the control group（ P = 0. 047）,while no difference was found in LXT group. Conclusion Longdan Xiegan Tang can efficiently alleviate anterior chambe

关 键 词：龙胆泻肝汤 实验性自身免疫性葡萄膜炎 炎症 组织病理学 房水蛋白 白蛋白 球蛋白 

分 类 号：R773.9[医药卫生—眼科]