LPS对5-FU杀伤QBC939细胞影响的实验研究  

Experimental Study of Effect of Combined with 5-FU on Killer Cells of QBC939

作　　者：霍胜军[1] 吕忠诚[1] 邹晓鹤[1] 宋建文[1] 王康[1] 

机构地区：[1]湖南省湘潭市第一人民医院普外三科,411101

出　　处：《医学研究杂志》2015年第3期78-80,共3页Journal of Medical Research

基　　金：江西省卫生厅基金资助项目(20091198)

摘　　要：目的探讨LPS联合化疗药物5-FU对人胆管癌QBC939细胞生长和诱导细胞凋亡的影响。方法四甲基唾哇蓝(MTT)试验检测LPS对5-FU抑制QBC939细胞增殖的影响,流式细胞术观察LPS对5-FU诱导QBC939细胞凋亡的影响。结果 1MTT法检测显示5-FU对胆管癌细胞株QBC939杀伤作用明显,但易出现耐药性,LPS可以增强5-FU后期杀伤作用,能一定程度上防止耐药的出现;2流式细胞术结果显示化疗药物5-FU后能显著诱导QBC939细胞发生凋亡,凋亡指数为11.50±2.15(P<0.05),对照组为3.53±0.32,加用LPS后,能更明显地诱导凋亡,凋亡指数达到26.50±3.12,与单用加化疗药物5-FU比较,差异有统计学意义(P<0.05)。结论 LPS能增强5-FU对胆管癌细胞株QBC939的后期杀伤作用,LPS联合化疗药物可能是胆管癌临床治疗的一种合理策略。Objective To explore the effect of LPS lombined with chemotherapy drugs 5 - FU on QBC939 human cholangiocarcino- ma cell growth and induced cell apoptosis. Methods Tetramethyl spit wow blue （MTT） assay was used to obserre that LPS inhibited QBC939 cell proliferation by 5 - FU. Flow eytometry was used to study the LPS on 5 - FU induced apoptosis QBC939. Results ①MTT assay showed that 5 - FU QBC939 ceils killing effect was obvious, but prone to drug resistance. LPS could enhance the killing effect of 5 - FU late, to prevent the emergence of resistance to a certain extent. ②Flow cytometry showed that the chemotherapy drug 5 - FU could significantly induce apoptosis in cells QBC939. Apoptotic index was 11.50 ± 2.15 （ P 〈 0.05 ）, （ the control group was 3.53 ± 0.32）. When plusing LPS, apoptosis was more obvious,and apoptotic index reached 26.50 ± 3.12. Compared with chemotherapy alone plus 5 - FU, the difference was statistically significant （P 〈 0.05）. Conclusion LPS can enhance the killing effect of 5 - FU late cholangiocarci- noma cell line QBC939. LPS bile duct cancer chemotherapy drugs may be a reasonable elinieal strategy.

关 键 词：胆管癌QBC939 CXCR4 脂多糖 基因治疗 

分 类 号：R73[医药卫生—肿瘤]