百里醌诱导膀胱癌细胞凋亡作用机制的研究  

Effects of Thymoquinone on Inducing Bladder Cancer BIU-87 Apoptosis

作　　者：王大文[1] 朱诗建 木海琦[1] 李晟[1] 周鹏飞[1] 杨森[1] 南存金[1] 陈映鹤[1] 

机构地区：[1]温州医科大学附属第二医院,325000

出　　处：《医学研究杂志》2015年第3期93-96,共4页Journal of Medical Research

基　　金：浙江省中医药科技计划基金资助项目(2011ZB092);浙江省卫生厅基金资助项目(2012KYB134)

摘　　要：目的探讨百里醌对人膀胱癌细胞株BIU-87细胞凋亡的影响及对其诱导凋亡的潜在作用机制。方法应用CCK8法检测细胞增殖活性,Hoechst33258染色法检测细胞凋亡,荧光定量PCR检测基因Bcl-2、Bax、caspase-3、c-myc mRNA的表达水平,Wsetern blot法检测不同浓度百里醌作用后Bcl-2、Bax、c-myc蛋白表达水平的变化。结果细胞增殖抑制曲线结果显示百里醌能明显抑制BIU-87细胞增殖,其24、48、72h半数抑制浓度(IC50)分别是38.75±0.58、34.33±1.01和32.43±0.71μmol/L。百里醌能以剂量依赖性方式诱导膀胱癌细胞凋亡。百里醌作用于BIU-87细胞后,Bcl-2、c-myc mRNA及Bcl-2、c-myc蛋白的表达量呈浓度依赖性降低,而caspase-3、Bax mRNA及caspase-3、Bax蛋白表达水平呈浓度依赖性递增。结论百里醌能抑制BIU-87细胞的增殖,且能诱导BIU-87细胞的凋亡,其诱导凋亡机制可能与Bcl-2、c-myc表达水平降低及caspase-3、Bax表达水平上调有关。Objective To investigate the effect of thymoquinone（TQ） on the human bladder cancer cell lines （ BIU - 87） apoptosis and its possible mechanisms of inducing apoptosis. Methods Cell proliferation ability was calculated by cell counting kit - 8 （ CCK8 ）. Hoechst staining was used to observe the apoptosis of BIU - 87 cells, treated with different concentrations of TQ. The mRNA expressions of Bcl - 2, Bax, c - myc and caspase - 3 were detected by real time fluorescent quantitative PCR. The expressions of Bcl - 2,bax and c - myc proteins were determined by Western blot. Results The results showed that TQ remarkably inhibited the BIU -87 cell proliferation with the IC^0 of 24h,48h and 72h respectively were 38.75 ± 0.58,34.33 ± 1.01,32.43 ± 0. 711μmol/L. Apoptosis was induced after trea- ted with TQ. Compared with the blank control group, the mRNA and proteins expressions of Bcl - 2 and e - mye obviously decreased in a dose - dependent manner, while the expressions of bax and caspase - 3 were upregulated. Conclusion TQ could significantly inhibit the proliferation ability of BIU - 87 cells, and also induce apoptosis probably by upregulating the expressions of Bax and easpase - 3 and downregulating the expressions of Bcl - 2 and c - myc.

关 键 词：百里醌 膀胱癌 BCL-2 BAX c—myc CASPASE 

分 类 号：R694[医药卫生—泌尿科学]