丝素蛋白/左旋聚乳酸支架材料与软骨细胞体外细胞相容性研究  

Cytocompatibility Study of Silk Fibroin/poly-L-lactic Acid Scaffold and Chondrocytes in Vitro

作　　者：孙莹[1] 李正强[1] 刘炜炜[1] 郑璐[2] 韩冰[1] 

机构地区：[1]吉林大学口腔医院口腔颌面外科,吉林长春130021 [2]吉林大学化学学院应用化学

出　　处：《口腔医学研究》2015年第3期221-225,共5页Journal of Oral Science Research

基　　金：吉林省科技厅科研基金资助课题(3D512K513431)

摘　　要：目的:观察静电纺丝支架材料丝素蛋白/左旋聚乳酸(SF/PLLA)的体外细胞相容性,探索其作为软骨组织工程支架材料的可行性,为进一步的体内及动物实验奠定基础。方法:将兔膝关节软骨细胞与丝素蛋白/左旋聚乳酸(SF/PLLA)支架材料复合培养,在第3、7、14天分别作HE染色和阿利新蓝+核固红染色,扫描电镜检验细胞黏着情况,MTT试验检测细胞在支架上的增殖情况。结果:细胞在支架上可以获得良好的粘附,细胞增殖良好,无细胞表型的变化。结论:丝素蛋白/左旋聚乳酸(SF/PLLA)具有良好的细胞相容性,可作为软骨组织工程支架材料。Objective： To lay the foundation for further body and animal experiment, we observe the cytocompati- hility on electrostatic spinning scaffold of silk fibroin/poly--L--lactic acid （SF/PLLA） in vitro and explore the fea- sibility in cartilage tissue engineering scaffolds. Methods： The rabbit knee joint chondrocyte were co--cultured with silk fibroin/poly--L--lactic acid （SF/PLLA） scaffold. Hematoxylin--eosin staining and Alcian blue-- nuclear fast red staining were tested on day 3, 7 and 14 respectively. Cell adhesion was tested by scanning electron microscopy （SEM）. Cell proliferation was determined by MTT test. Results： Adhesion, proliferation, and growth of chondro- cyte derived from rabbit knee joint chondrocytes were great on the surface of silk fibroin/poly--L--lactic acid （SF/ PLLA）. The chondrocytes cultured in vitro maintained the specific chondrocytes phenotype on the scaffold. Conclu- sion： Silk fibroin/poly--L--lactic acid （SF/PLLA） scaffold exhibits excellent cytocompatibility, and can be used for cartilage tissue engineering.

关 键 词：丝素蛋白/左旋聚乳酸(SF/PLLA) 组织工程 软骨 

分 类 号：R783.1[医药卫生—口腔医学]