新型GBR胶原膜的早期动物体内植入实验研究  

Guided Bone Regeneration in Mandibular Defects Using a Novel Collagen Membrane

作　　者：白冰[1] 朱静涛[2] 冯丽芳[1] 宋荣博[3] 

机构地区：[1]中国医科大学口腔医学院修复科,辽宁省口腔医学研究所,辽宁沈阳110001 [2]中国医科大学口腔医学院奉天门诊 [3]中国医科大学口腔医学院中心实验室

出　　处：《口腔医学研究》2015年第3期226-229,233,共5页Journal of Oral Science Research

基　　金：辽宁省自然科学基金(2013021038)

摘　　要：目的：探讨新型国产GBR胶原膜体内植入后,诱导早期膜下成骨的能力。方法：实验于2013年10月~2014年3月在沈阳军区总医院动物实验中心完成。选取小型巴马猪,于双侧下颌骨骨体处用牙科裂钻制备8mm×8mm全层骨缺损3个,分别应用实验胶原膜、Bio-gide@、无覆盖膜覆盖骨缺损。术后1个月处死动物,分别在处死前1、2周分别肌肉注射四环素溶液与二甲酚橙溶液。固定样本后,制备硬组织切片。分别在荧光显微镜及光学显微镜下（甲苯胺蓝、亚甲基蓝-酸性品红染色）观测膜的降解程度及膜下新骨生成能力,评价材料膜下骨形成量和骨成熟程度。结果：实验组胶原膜具备良好的屏障作用,膜下新生骨矿化程度良好;骨小梁排列整齐,但新生骨量少于对照组。结论：新型国产胶原膜在1个月时无明显降解,具备良好的膜下成骨能力,需进行实验组胶原膜的改性,以增加膜下成骨量。Objective： To evaluate a new synthetic degradable barrier membrane based on collagen for use in guided bone regeneration. Methods： The collagen membrane was embedded in Bama pigs mandibular bone and Bio--gide@ membrane served as reference materials. In Bama pigs, three standardized 8min×8,m defect was created in man- dibular bone. Three groups （collagen, Bio--gide@, no membrane） were evaluated at 4 weeks. New bone formation was demonstrated by histological analysis. Results： In the Bio--gide@ and collagen groups, the defects were incom- pleted new formedbone filling. In contrast to uncovered mandibular defects, substantial bone healing was observed in defects covered with a barrier membrane. In the non membrane covered case, the formation of bone was un- formed in 4 weeks. There were new formed bone under the Bio--gide@ and collagen membranes. The amount of new bone formed under the Bio--gide@ membranes were more than the amount of bone formed under the collagen membranes. Conclusion： It can be concluded that the collagen membrane was well suited for use in guided bone re- generation.

关 键 词：胶原膜 引导骨再生 新骨生成 

分 类 号：R783.6[医药卫生—口腔医学]