血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响  

Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113

作　　者：周彩然 郭淑娟[2] 曾锦[2] 黄海森[2] 姜思聪[2] 田卫东[2,3] 邱嘉旋[1] 

机构地区：[1]南昌大学第一附属医院口腔颌面外科,江西南昌330006 [2]四川大学口腔再生医学国家地方联合工程实验室 [3]四川大学华西口腔医院口腔颌面外科

出　　处：《口腔医学研究》2015年第3期254-257,共4页Journal of Oral Science Research

基　　金：江西省自然科学基金项目(编号:20114BAB205055)

摘　　要：目的:研究血竭素高氯酸盐(Dracorhodin perchlorate,DP)对舌鳞癌细胞Tca-8113增殖和凋亡的影响并初步探讨其相关机制。方法:以舌鳞癌细胞Tca-8113为研究对象,采用CCK-8、流式细胞术分析及Western Blot等方法检测不同浓度DP对Tca-8113细胞的增殖、凋亡及核因子E2-相关因子2(nuclear factor E2-related factor 2,Nrf2)、血红素氧合酶1(heme oxygenase 1,HO-1)蛋白表达的变化。采用SPSS17.0统计软件对实验结果进行分析。结果:CCK-8检测表明,与空白对照组相比,DP可以显著抑制细胞增殖,且对细胞的增殖抑制作用有时间-剂量依赖性(P<0.05)。流式细胞术及Western Blot检测结果显示DP可以促进细胞凋亡(P<0.05),并提高Nrf2、HO-1在Tca-8113细胞中的表达。结论:DP能抑制舌鳞癌细胞Tca-8113增殖并诱导其发生凋亡,其作用可能与Nrf2/HO-1信号通路激活有关;可能成为抗口腔癌药物。Objective.. To investigate the effect of dracorhodin perchlorate （DP） on the cell proliferation and apop- tosis and to discuss relevant signaling pathways in human tongue squamous carcinoma cell line Tca--8113 . Meth- ods： The Tca8113 cells were used as research object in this experiment. The growth, apoptosis and the expression of nuclear factor E2--related factor 2 （Nrf2） and heine oxygenase 1 （HO--1） were detected in human tongue carci- noma cell line（Tca--8113）, using cell counting kit--8 （CCK--8）, the flow cytometry and Western blot to assay. All statistical analysis was performed using the SPSS 17.0 statistical package. Results： CCK--8 assay proved that there was a much pronounced decrease in cell viability on treatment with DP compared with control group and the time-- and dose--dependent effects of treatment with DP on the growth of cells （P〈0.05）. Flow cytometric analy- sis and Western blot demonstrated that DP promoted cell apoptosis （P〈0.05） and elevated the expression of Nrf2 and HO--1 proteins in Tca--8113 cells. Conclusion： DP inhibited tongue squamous carcinoma Tca--8113 cells growth and induced apoptosis, which may be relevant with the activation of Nrf2/HO--1signaling pathway in oral cancer cells. DP could be developed as an agent against OSCC.

关 键 词：血竭素高氯酸盐 Nrf2/HO-1 TCA-8113 凋亡 

分 类 号：R739.86[医药卫生—肿瘤]