牙龈卟啉单胞菌脂多糖(Pg-LPS)对破骨细胞EphA2基因表达的调控  

Effect of Porphyromonas Gingivalis Lipopolysaccharide(Pg-LPS)on the Expression of EphA2 Gene in Osteoclast

作　　者：高爱超[1] 于海燕[1] 李娜[1] 侯玉帛 戚欣[2] 于小琳[3] 于维先[1,3] 

机构地区：[1]吉林大学口腔医学院牙周病科,吉林长春130021 [2]北华大学 [3]吉林大学口腔医学院,吉林省牙发育及颌骨重塑与再生重点实验室

出　　处：《口腔医学研究》2015年第3期268-271,275,共5页Journal of Oral Science Research

基　　金：吉林省科技厅自然科学基金资助课题(编号:201115104)

摘　　要：目的:研究小鼠单核巨噬细胞系RAW264.7在破骨分化过程中,Pg-LPS对破骨细胞EphA2表达的影响。方法:用终浓度10mg/L的Pg-LPS刺激RAW264.7细胞后,分别在1、3、5d,应用RT-PCR检测破骨细胞中EphA2基因和破骨细胞相关基因(破骨细胞内基质金属蛋白酶(MMP9)、ACP5、c-fos、组织蛋白酶K(CtsK)、NFATc1)的表达,并且通过酒石酸抗酸性磷酸酶(TRAP)染色观察实验组和对照组破骨细胞的分化成熟情况。结果:RT-PCR检测10mg/L的Pg-LPS在第3天和5天,实验组比对照组EphA2基因表达分别增高2.4倍和1.2倍,两组之间存在显著差异(P<0.01);同时也能够促进破骨相关基因c-fos、NFATc1、CtsK、ACP5、MMP9的表达,实验组与对照组相比差异有显著性;TRAP染色结果显示:实验组比对照组的TRAP阳性多核细胞数目明显增多。结论:10mg/L的Pg-LPS对小鼠单核巨噬细胞系RAW264.7,在破骨分化的中期和晚期均能够促进EphA2基因的表达,但是在破骨分化早期对EphA2基因的表达无明显作用。Objective： To investigate the effect of porphyromonas gingivalis lipopolysaccharide （Pg--LPS） on the expression of EphA2 gene in RAW264.7 cells during osteoclastic differentiation. Methods： RAW264. 7 cells were stimulated with 10t^g/ml of porphyromonas gingivalis lipopolysaccharide （Pg--LPS） at 1, 3 and 5 d. RT--PCR was applied to determine the expression of EphA2 gene and the osteoclast related genes （MMPg, c -- fos, ACP5, CtsK,NFATcl）. Tartrate--resistant acid phosphatase （TRAP） staining was applied to observe osteoclast differen- tiation and maturation. Results： Compared with the control group, at the 3 d and 5 d, the EphA2 gene mRNA ex- pression was significantly increased 2.4--fold and 1.2--fold in Pg--LPS group. At the I d, there was no obvious difference between the Pg-- LPS group and control group. Meanwhile Pg-- LPS stimulation significantly promote osteoclast related genes of c--los, NFATcl, CtsK, ACP5 and MMP9 expressions. Tartrate--resistant acid phos- phatase （TRAP） staining showed that compared with the control group, the number of the TRAP positive cells was significantly increased in the Pg--LPS group. Conclusion： Pg--LPS can promote the expression of EphA2 gene in the middle and later stages of osteoclast differentiation,but has no obvious effect in the early stage.

关 键 词：Pg-LPS 破骨细胞 EphA2基因 

分 类 号：R780.2[医药卫生—口腔医学]