Role of adsorbed proteins on hydroxyapatite-coated titanium in osteoblast adhesion and osteogenic differentiation  

羟基磷灰石涂层修饰钛表面的蛋白质吸附及其对成骨细胞黏附和分化的作用(英文)

作　　者：吴赛 刘宣勇 高长有 

机构地区：[1]Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University

出　　处：《Science Bulletin》2015年第7期691-700,M0003,共11页科学通报（英文版）

基　　金：supported by the National Basic Research Program of China(2011CB606203);the National Natural Science Foundation of China(21434006,21374097)

摘　　要：The method of plasma-spray coating of hy- droxyapatite （HA） onto pure titanium has been demon- strated to be effective to enhance the osteogenic differentiation and accelerate bone regeneration. Yet it is still a big challenge to figure out the interplay among im- plant surface properties, adsorbed proteins and cell-surface interactions. In this study, the plasma-sprayed HA-coated titanium （HA-Ti） surface was compared with the titanium substrate in terms of protein adsorption, cell adhesion and differentiation. The phase composition, wettability and to- pography were characterized. Compared to the Ti substrate, the HA-Ti had a smaller water contact angle, but larger micro-scale roughness, and showed a poorer ability to ad- sorb fibronectin （Fn）, bovine serum albumin （BSA） and serum proteins. However, it could adsorb larger amount of recombinant human bone morphogenetic protein 2 （BMP- 2）. The osteoblasts and bone marrow mesenchymal stem cells （BMSCs） tended to adhere on the Ti substrate. By contrast, the BMSCs cultured on the HA-Ti showed a stronger tendency toward osteogenesis differentiation.通过等离子体喷涂的方法在纯钛表面修饰羟基磷灰石涂层,可有效促进成骨分化并加速骨再生.生物材料表面的蛋白质吸附对后续细胞行为起着至关重要的作用,然而材料表面性能-蛋白质黏附-细胞行为之间的内在关系仍有待研究和阐明.本文检测了羟基磷灰石涂层(HA-Ti)和纯钛表面(Ti)吸附蛋白质层的性质,研究了蛋白质层对两种材料表面细胞黏附状态、死活细胞数和成骨分化的影响.相比Ti表面,HA-Ti表面纤维黏连蛋白(Fn)和血清蛋白吸附量较少,成骨细胞和干细胞黏附数量少;但HA-Ti表面对人成骨细胞BMP-2的吸附量稍大,诱导成骨分化明显.这说明HA涂层表面对蛋白质吸附性能的差异会导致细胞响应的不同,进而影响其生物学功能.

关 键 词：HYDROXYAPATITE IMPLANTS Proteinadsorption FIBRONECTIN Bone morphogeneticprotein - Mesenchymal stem cells 

分 类 号：R318.08[医药卫生—生物医学工程]