黄芩素对HT-29细胞增殖、迁移的影响及相关机制研究  

Effect and mechanism of scutellarein in suppressing the migration and proliferation of colorectal cancer HT- 29 cells

作　　者：赵晓越[1] 王杰军[2] 于观贞[2] 王湛 焦晓栋[2] 柳珂[2] 江龙委[2] 

机构地区：[1]解放军八一医院全军肿瘤中心肿瘤内科,210002 [2]第二军医大学长征医院肿瘤科,上海200433

出　　处：《临床肿瘤学杂志》2015年第3期208-211,共4页Chinese Clinical Oncology

摘　　要：目的探讨黄芩素对结肠癌HT-29细胞增殖、迁移能力的影响。方法 MTT法观察黄芩素(5、10、20、40、80μg/ml)对HT-29细胞的增殖抑制作用,Transwell试验评价黄芩素(80μg/ml)对HT-29细胞迁移能力的抑制作用,Western blotting检测黄芩素(20、40μg/ml)对HT-29细胞中信号转导与转录激活因子3(STAT3)、核因子(NF)-κB和p53蛋白表达的影响。同时以未加黄芩素为对照组。结果与对照组比较,黄芩素对细胞增殖能力有抑制作用,增殖抑制率随浓度升高而增加,差异均有统计学意义(P<0.05);黄芩素处理后的细胞迁移能力及STAT3和NF-κB蛋白水平均降低,p53蛋白水平升高,与对照组的差异均有统计学意义(P<0.05)。结论黄芩素可显著抑制HT-29细胞的增殖、迁移能力,可能与上调细胞中p53、降低STAT3和NF-κB蛋白的表达有关。Objective To explore the effect of scutellarein on the migration and proliferation of colorectal cancer HT-29 cells as well as the underlying mechanism. Methods The MTT assay was used to investigate the proliferation of HT-29 cells after treatment with different doses of scutellarein（5, 10, 20, 40, 80 μg/ml）. The effect of 80 μg/ml scutellarein on migration of HT-29 cells was measured by Transwell assay. Protein expression of signal transducer and activator of transcription（STAT3）, nuclear factor（NF）-κB and p53 in HT-29 cells was assayed by Western blotting after treatment of 20, 40μg/ml scutellarein. Cells without treatment were as-signed as control group（ 0 μg/ml scutellarein） . Results Compared with the control group, scutellarein presented inhibitory effect on the proliferation ability of T29 cells in a dose-dependent manner with significant difference（ P〈0.05） . There were decreased cell migra-tion ability and protein levels of STAT3 and NF-κB but increased level of p53 in scutellarein group versus control group（P〈0.05）. Conclusion Scutellarein significantly inhibited the migration and proliferation of HT-29 cells with the possible mechanism of increasing expression of p53 and decreasing levels of STAT3 and NF-κB in HT-29 cells.

关 键 词：黄芩素 结肠癌 增殖 迁移 

分 类 号：R735.3[医药卫生—肿瘤]