小细胞肺癌组织细胞周期相关激酶的表达及其临床意义  

Expression of CCRK in small cell lung cancer and its clinical significance

作　　者：邵泽伟[1] 山凤莲[2] 孙炜炜[1] 李雷生[1] 聂尚丹[1] 张孝霞[1] 郑红[1] 郭其森[3] 

机构地区：[1]济宁医学院医学检验系临床血液学教研室,山东省济宁272067 [2]济宁医学院附属医院呼吸内一科 [3]山东省医学科学院附属肿瘤医院内一科

出　　处：《中国医师杂志》2015年第3期343-346,共4页Journal of Chinese Physician

基　　金：山东省自然基金资助项目（ZR2012HL36）

摘　　要：目的：探讨细胞周期相关激酶（ CCRK）在小细胞肺癌组织中的表达及意义。方法采用RT－PCR法和Western印迹法分别从mRNA水平和蛋白质水平检测CCRK在小细胞肺癌和非肺癌组肺组织中的表达。结果小细胞肺癌组织中CCRK mRNA [（0．51±0．11） IU／L ]及蛋白水平[（0．61±0．13）IU／L]均明显高于非肺癌组肺组织CCRK mRNA[（0．30±0．08） IU／L]及蛋白水平[（0．34±0．09）IU／L]，差异具有统计学意义（ P ＜0．05）；CCRK mRNA和CCRK蛋白的表达与化疗疗效有关（ P ＜0．05），而与临床分期无关（ P ＞0．05）。结论 CCRK在小细胞肺癌组织中呈高表达，对小细胞肺癌的发生发展具有促进作用；化疗可以有效地抑制CCRK基因的过表达，不同疗效组化疗前后CCRK mRNA和CCRK蛋白的表达差异显著。Objective To investigate the pathogenesis of small cell lung cancer （ SCLC） and to ex-plore the expression of cell cycle related kinase （ CCRK） in SCLC and its clinical significance.Methods Reverse transcription polymerase chain reaction （ RT-PCR） and Western blot were performed to examine ex-pression of CCRK in SCLC and normal tissues.Results The expressions of gene [（0.51 ±0.11）IU/L] and protein [（0.61 ±0.13）IU/L] of CCRK in SCLC tissues were significantly higher than normal tissues [（0.30 ±0.08）IU/L, （0.34 ±0.09）IU/L] （ P 〈0.05）.The expression of CCRK was closely correlated with the clinical curative effect （ P 〈0.05 ） rather than the clinical stages （ P 〉0.05 ） .Conclusions The expressions of gene and protein of CCRK in SCLC tissues were significantly higher than normal tissues. CCRK promoted the occurrence and progress of SCLC.Chem can restrain effectually the excessive expres-sion of CCRK.The expressions of gene and protein of CCRK in the different clinical curative effect group had significant difference.

关 键 词：肺肿瘤/代谢 细胞周期蛋白质依赖激酶类/代谢 

分 类 号：R734.2[医药卫生—肿瘤]