PI3 K、Akt 及 E－cadherin 在甲状腺乳头状癌中的表达及其临床意义  

Expression and clinical significance of PI3K, Akt and E-cadherin in thyroid papillary carcinomas

作　　者：薛栋[1] 孔丽[2] 左凯[3] 李新军[4] 张同军[1] 

机构地区：[1]滨州市人民医院肝胆外科,山东省滨州256610 [2]滨州市人民医院静配中心,山东省滨州256610 [3]滨州市人民医院感染科,山东省滨州256610 [4]滨州市人民医院病理科,山东省滨州256610

出　　处：《中国医师杂志》2015年第3期357-360,共4页Journal of Chinese Physician

基　　金：山东省滨州市科技发展计划项目（2011ZC0934）

摘　　要：目的：探讨磷脂酰肌醇－3－激酶（PI3K）、蛋白激酶B（Akt）及E－钙黏素（E－cadherin）在甲状腺乳头状癌中的表达及其临床意义。方法应用免疫组织化学EnVison法检测62例甲状腺乳头状癌、30例结节性甲状腺肿、30例正常甲状腺组织中PI3K、Akt及E－cadherin的表达，并对性别、年龄、肿瘤大小、TNM分期及淋巴结转移等因素进行分析。结果62例甲状腺乳头状癌中PI3K、Akt及E－cadherin蛋白的阳性表达率分别为74．2％（46／62）、66．1％（41／62）、16．1％（10／62） ，与结节性甲状腺肿、正常甲状腺组织相比，差异均有统计学意义（ P ＜0．05）。甲状腺乳头状癌Ⅰ-Ⅱ期中PI3K及Akt蛋白的阳性表达率均低于Ⅲ-Ⅳ期（χ2＝4．976， P ＝0．026；χ2＝6．233， P ＝0．013）；淋巴结转移组中PI3K及Akt蛋白的阳性表达率均高于淋巴结无转移组（χ2＝6．675， P ＝0．010；χ2＝7．511， P ＝0．006）。而E－cadherin蛋白在Ⅰ-Ⅱ期的阳性表达率明显高于Ⅲ-Ⅳ期（χ2＝6．558， P＝0．010）；E－cadherin蛋白在淋巴结无转移组的阳性表达率明显高于伴有淋巴结转移组（χ2＝5．678， P ＝0．017），经Spearman等级相关性分析表明，PI3K与Akt蛋白的阳性表达呈正相关（ r ＝0．423， P＜0．05），PI3K与E－cadherin蛋白的阳性表达呈负相关（ r ＝－0．527， P ＜0．05），Akt与E－cadherin的阳性表达也呈负相关（ r ＝－0．417， P ＜0．05）。结论 PI3K／Akt信号通路参与了甲状腺乳头状癌的发展、侵袭及转移。Objective To investigate the expressions of phosphatidylinositol 3-kinase （PI3K）, Akt and E-cadherin and their clinical significance in thyroid papillary carcinomas.Methods Expressions of PI3K, Akt, and E-cadherin were detected in 62 cases of thyroid papillary carcinomas,30 cases of thyroid goiter and 30 cases of normal thyroid by immunohistochemistry （EnVison）,and simultaneously compared with age, sex, tumor size, clinical tumor node metastasis（ TNM） stages, and lymph node metastasis in thy-roid papillary carcinomas.Results The expression rate of PI3K, Akt, and E-cadherin was 74.2%（46/62）, 66.1%（41/62）, 16.1%（10/62）,respectively.Expressions of three proteins in thyroid papillary carcinomas were significantly different from those in thyroid goiter and normal thyroid tissues （ P 〈0.05）. The lower positive rates of PI3K and Akt proteins were obtained in the group of stageⅠ-Ⅱthan that in the group of stageⅢ-Ⅳ（χ2 =4.976, P =0.026;χ2 =6.233, P =0.013）.Higher positive rates of PI3K and Akt proteins were obtained in the group of lymph-node metastasis than that in group of non-lymph-node metastasis （χ2 =6.675, P =0.010;χ2 =7.511, P =0.006）.Higher positive rate of E-cadherin protein was obtained in the group of stage Ⅰ-Ⅱ than that in the group of stage Ⅲ -Ⅳ （χ2 =6.558, P =0.010 ） .Higher positive rate of E-cadherin proteins was obtained in the group of non-lymph-node metastasis＆amp;nbsp;than that in the group of lymph node metastasis（χ2 =5.678, P =0.017）.There was significant positive correlation between expressions of PI3K and Akt through Spearman correlation analysis （ r =0.423, P 〈0.05）.PI3K was negatively correlated with E-cadherin with Spearman correlation analysis （ r =-0.527, P 〈0.05）.Akt was also negatively correlated with E-cadherin （ r =-0.417, P 〈0.05）.Conclusions PI3K/Akt pathway might regulate thyroid papillary carcinoma cells proliferation, invasion and metastasis.

关 键 词：甲状腺肿瘤/代谢 癌 乳头状/代谢 1-磷脂酰肌醇3-激酶/代谢 蛋白激酶类/代谢 钙黏着糖蛋白类/代谢 

分 类 号：R736.1[医药卫生—肿瘤]