白花蛇舌草多糖诱导鼻咽癌细胞凋亡及其机制研究  

Mechanism of apoptosis of nasopharyngeal carcinoma cells induced by polysaccharides extracts from Hedyotic diffusa

作　　者：景艳[1] 康敏[2] 刘津[3] 李静雨[4] 唐安洲[4] 

机构地区：[1]广西壮族自治区人民医院耳鼻咽喉科南宁,530021 [2]广西医科大学第一附属医院放疗科 [3]右江民族医学院附属医院耳鼻咽喉科 [4]广西医科大学第一附属医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第7期641-644,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的：研究白花蛇舌草多糖（PEHD）体外抑制鼻咽癌CNE2细胞株增殖,诱导细胞凋亡及其凋亡机制。方法：用不同剂量PEHD（2、4、6mg/ml）处理CNE2细胞24h、48h、72h,用四甲基偶氮唑蓝法（MTT）检测CNE2细胞的增殖情况,计算抑制率。在不同药物浓度（2、4、6mg/ml）PEHD作用48h后,用Annexin V-FITC/碘化丙锭双染法（Annexin V/PI）标记的流式细胞术检测CNE2细胞的凋亡率。采用Western blot检测用药前后细胞中Bax蛋白、Bcl-2蛋白和caspase-3蛋白的表达水平。结果：MTT结果显示,2、4、6mg/ml PEHD可以明显抑制CNE2细胞增殖（均P〈0.05）,最高抑制率可达76.5%,在2~6mg/ml浓度内随着浓度的增加、时间的延长抑制作用逐渐增强,呈时间-剂量依赖性。流式细胞术检测到4、6mg/ml PEHD作用48h后,CNE2凋亡细胞比例显著增加,凋亡率分别为31.32%、46.28%,高于空白对照组4.86%（P〈0.01）。Western blot显示PEHD处理48h后,CNE2细胞Bax蛋白和caspase-3蛋白表达上升,Bcl-2的表达下降。结论：在一定浓度范围内PEHD（2、4、6mg/ml）能够明显抑制鼻咽癌CNE2细胞的增殖,呈时间-剂量依赖性,其抑制作用与诱导细胞凋亡有关;PEHD可通过上调Bax、caspase-3蛋白表达、下调Bcl-2蛋白表达,诱导CNE2细胞凋亡。Objective：To explore the proliferation inhibition and apoptosis of polysaccharides extracts from polysaccharides extracts from Hedyotic diffusa（PEHD）on Human Nasopharyngeal Carcinoma（NPC）cell line CNE2 cells in vitro.Method：CNE2cells treated with various concentrations of PEHD were detected by MTT assay at 24 h,48h,and 72 h.The apoptotic cells were analyzed by flow cytometry with Annexin V/PI staining.The expression levels of Bax,Bcl-2and caspase-3protein were examined by Western blotting method.Result：The growth of CNE2 cells were suppressed after treatment with PEHD（P〈0.05）,MTT assay showed that the highest cell inhibition rate reached to 76.5%,the inhibition in the doses from 2to 6mg/ml showed dose-and-time-dependent.The percent of apoptosis in 4and 6mg/ml PEHD treatment groups for 48 hwere 31.32%,46.28%,respectively,and significantly higher than that in control groups,4.86%（P〈0.01）.After the cells being treated with PEHD for 48 h,the expression of Bax and caspase-3protein increased,and the expression of Bcl-2protein decreased gradually.Conclusion：PEHD could inhibited the growth of CNE2 cells and was dose-and-time-dependent,the mechanism may involve induction of cell apoptosis,which was associated with the activation of Bax and caspase-3protein and the down-regulation of Bcl-2protein expression.

关 键 词：白花蛇舌草多糖 鼻咽肿瘤 CNE2细胞 细胞凋亡 

分 类 号：R739.63[医药卫生—肿瘤]