rhBMP-2对大鼠成骨细胞PKA的作用研究  

Effect of rhBMP-2 on Protein Kinase A of osteoblasts in SD rat

作　　者：葛成[1] 孙海燕[1] 李齐宏[1] 于开涛[1] 舒瑶[1] 刘宝林 杨连甲 

机构地区：[1]中国人民解放军第三0七医院口腔科,北京100071 [2]第四军医大学口腔医院

出　　处：《中国美容整形外科杂志》2015年第4期220-223,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金资助项目（30070818）;北京市自然科学基金资助项目（7152110）

摘　　要：目的探讨rhBMP-2对大鼠成骨细胞PKA的影响。方法首先，分别以rhBMP4（200μg/L）、PKI—rhBMP4（1mg/L的PKI预刺激30An后加rhBMP4）干预大鼠成骨细胞0、10、30、60min，检测PKA的活性；然后，检测在200μg/L的rhBMP-2分别作用10、20、30min后，大鼠成骨细胞的cAMP浓度；再通过RT-PCR方法，检测rhBMP-2分别作用0（con）、30．60rain后，细胞内PKAa及PKABmRNA。结果rhBMP．2干预成骨细胞30、60min后，PKA活性升高（P〈0．05），PKI预刺激30min后，细胞PKA活性显著降低（P〈0．01），rhBMP-4使已经降低的PKA活性逐渐升高，尤其是在加入rhBMP460min后（P〈0．01）。rhBMP-2干预成骨细胞10、20、30min后，细胞内的cAMP浓度无明显变化（P〉0．05）。rhBMP-2对成骨细胞PKAa及PKABmRNA的影响很小，在60min之内，无显著变化（P〉0．05）。结论rh—BMP-2可以直接或者通过CAMP以外的某个途径，促进体外培养的大鼠成骨细胞内PKA的活性，对PKA的合成代谢在短期内（60min内）无明显影响。Objective To explore the effect of rhBMP-2 on PKA activity of osteoblasts in SD rat. Methods Firstly, PKA activities were measured after the cells were treated with rhBMP-2 （200 μg/L） and PKI-rh-BMP-2 （ 1 mg/L PKI pretreating for 30 minutes ） for 0, 10, 30 and 60 minutes respectively. Then, the cAMP concentration was detected after the cells were treated with rhBMP-2 for 10, 20, 30 minutes and PKAα and PKAβ mRNA was also measured byRT-PCR after tile cells were treated with rhBMP-2 for 0, 30, 60 minutes. Results The PKA activity was increased by rhBMP-2 treating for 30 and 60 minutes （ P 〈 0.05 ）. PKA activity was decreased after PKI pretreated for 30 minutes （ P 〈 0.01 ）, whereas rhBMP-2 elevated the depressed PKA activity in a time-dependent manner （ P 〈 0. 05 ）. No difference of the amount of cAMP was found between the experimental group （treated with rhBMP-2 for 10, 20, 30 minutes respectively） and the control group （ P 〉 0.05 ）. PICA mRNA was detected through RT-PC within 60 minutes, there was no significant effect of rhBMP- 2 on rat cells PKA mRNA level （ P 〉 0.05 ）. Conclusion PKA activity in SD rat osteoblasts can be increased by rhBMP-2 directly or by some pathway except for cAMP signal pathway. And rhBMP-2 does not affect PKA synthesis （ mRNA level） in the exposure time less than 60 minutes.

关 键 词：骨形态发生蛋白 蛋白激酶A 成骨细胞 信号通路 

分 类 号：R-332[医药卫生]