侧脑室微量注射瘦素对大鼠下丘脑GnIH表达的影响  

EFFECTS OF MICROINJECTION OF LEPTIN INTO LATERAL VENTRICLE ON GNIH EXPRESSION IN RATS' HYPOTHALAMUS

作　　者：魏萌[1] 武俭[1] 苏玮[1] 杨松鹤[1] 程露阳[1] 乔跃兵[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2015年第2期91-93,共3页Journal of Chengde Medical University

基　　金：河北省自然科学基金项目(H2013406115);河北省卫生厅医学科学研究项目(20130012);河北省人口计生委科学研究项目(2012-A22);河北省高校重点发展学科"人体解剖与组织胚胎学"建设项目资助

摘　　要：目的:探讨侧脑室微量注射瘦素对下丘脑促性腺激素抑制激素(GnIH)蛋白表达的影响。方法:10只雌性Wistar大鼠随机分为注射生理盐水6h组和注射瘦素6h组,每组5只大鼠。大鼠去卵巢手术同时给予17β-雌二醇后,分别于侧脑室微量注射生理盐水和瘦素各4μl。采用蛋白印迹技术检测下丘脑GnIH蛋白表达的变化。结果:与注射生理盐水6h组相比,微量注射瘦素可明显降低下丘脑GnIH的表达水平(P<0.05)。结论:瘦素可能通过抑制GnIH的表达发挥对下丘脑-垂体-性腺轴的调节作用。Objective：To explore the effects of microinjection of leptin into lateral ventricle on gonadotropin-inhibitory hormone（Gn IH） expression in rats＇ hypothalamus. Methods： 10 female Wistar rats were randomly divided into injection of normal saline 6h group and injection of leptin 6h group（n=5）. The rats in 2 groups were respectively injected 4μl normal saline and 4μl leptin into the lateral ventricle after bilateral ovariectomy and injecting 17β-estradiol. Western blot was used to detect the expression of Gn IH in rats＇ hypothalamus. Results： Compared with rats in injection of normal saline 6h group, the expression of Gn IH in hypothalamus of rats in injection of leptin 6h group reduced obviously（P〈0.05）. Conclusions： Leptin may play a role in regulating the hypothalamic-pituitary-gonadal axis by inhibiting the expression of Gn IH.

关 键 词：侧脑室 瘦素 GNIH 下丘脑-垂体-性腺轴 

分 类 号：R338.2[医药卫生—人体生理学]