表面化学修饰纳米金粒子对肝癌细胞增殖和迁移的影响  

Effect of Gold(Au) Nanoparticles Modified by Surface Chemistry on the Proliferation and Migration of Hepatocellular Carcinoma Cells in Vitro

作　　者：洪锦勇 尹红梅[2] 沈阳[1] 闫志平[1] 刘静霞[1] 周法庭[1] 夏庆[1] 刘肖珩[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物医学工程研究室,成都610041 [2]四川大学华西药学院,成都610041

出　　处：《生物医学工程学杂志》2015年第2期373-379,共7页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(11372203;11172189);教育部高等学校博士学科点专项科研基金资助项目(20120181120058);国家基础科学人才培养基金能力提高资助项目(J1103604)

摘　　要：由于具有高强度的靶向性与优良的生物相容性等优势,纳米载药颗粒已被广泛应用于癌症的诊断和治疗中。然而当纳米颗粒被癌细胞识别并内吞后,其对癌细胞迁移行为的影响却鲜有报道。在本研究中,我们采用自组装技术(SAMs)成功制备了具有不同化学官能团(CH3、OH、COOH和NH2)的纳米金颗粒(Au NPs),通过透射电镜观察Au NPs在人肝癌细胞株HepG2细胞内的分布情况,采用MTT法检测其对该细胞增殖的影响,同时利用划痕损伤实验模型和Transwell小室模型法观察Au NPs对该细胞迁移的影响。结果显示,不同化学官能团修饰的纳米金颗粒被细胞内吞后主要分布在囊泡边缘或胞间连接处。此外,我们发现不同化学官能团修饰的纳米金均会对HepG2细胞的活性起到一定的抑制作用,且随着纳米金粒子浓度的升高,其细胞增殖能力逐渐降低。划痕模型和Transwell模型结果证实不同表面化学官能团修饰的纳米金颗粒对细胞迁移的影响均有差异,与对照组比较,NH2-NPs、OH-NPs组显著抑制了人肝癌细胞株HepG2的迁移行为。Due to the good tumor-targeting and excellent biocompatibility,the drug-loading nanoparticles（NPs）has been widely applied in the diagnosis and treatment of cancer.However,after the NPs are recognized and internalized by cancer cells,the effects of NPs on cell migration behavior were unclear.In the present study,the self-assembly techniques（SAMs）was used to modify gold（Au）nanoparticles（Au NPs）with different chemical functional groups（CH3,OH,COOH and NH2）as model NPs.The dispersion of these groups in solution and the distribution in cells were studied by transmission electron microscope（TEM）,respectively,and the proliferation was examined by MTT assay in vitro.The wound-healing and the Transwell assay were used to examine the effect of internalized Au-NPs on HepG2 cells migration.The results showed that different Au-NPs mainly distributed at the edge of the vesicle membrane and the gap between cells.The Au-NPs resulted in decreased cell viability in a concentration-depended manner.In addition,the results of wound-healing and Transwells assay indicated that the internalization of the NH2-NPs and OH-NPs would inhibit cell migration compared with those in the control group.

关 键 词：表面化学 纳米金 人肝癌细胞 细胞毒性 细胞迁移 

分 类 号：R735.7[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]