吉西他滨耐药的胰腺癌SW1990细胞株的建立及相关特性检测  

Establishment of the human resistant pancreatic cancer cell lines SW1990/gemcitabine and its characteristics test

作　　者：贾一夫[1] 丁飞[1] 余跃[1] 高萌[1] 杨显珠[2] 王均[2] 

机构地区：[1]安徽医科大学附属省立医院消化内科,合肥230001 [2]中国科学技术大学生命科学学院合肥微尺度物质科学国家实验室,合肥230027

出　　处：《安徽医科大学学报》2015年第4期419-422,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH174)

摘　　要：目的探讨对吉西他滨(GEM)耐药的胰腺癌SW1990细胞诱导方法,以期进一步认识胰腺癌耐药的发生机制,并对诱导的GEM耐药的胰腺癌细胞与其亲本细胞的生物学特性进行比较。方法采用逐步浓度递增法诱导对GEM耐药的细胞株SW1990-GZ。MTT法检测SW1990和SW1990-GZ的半数致死量(IC50)、耐药系数(R),并观察其生长差异。采用高效液相色谱法(HPLC)检测不同时间点SW1990和SW1990-GZ对GEM药物的摄取情况。结果SW1990和SW1990-GZ的IC50为(0.07±0.002 1)、(87.50±3.240 0)μg/ml,R=1 250;在不同浓度GEM作用下,诱导后耐药的SW1990-GZ对GEM的敏感性明显降低。细胞生长曲线显示耐药细胞SW1990-GZ相对于SW1990生长缓慢。不同时间点GEM孵育后,SW1990-GZ细胞对GEM药物的摄取较SW1990细胞降低。结论成功诱导了耐GEM药物的SW1990-GZ细胞株,耐药性能稳定、明显。SW1990-GZ细胞可能存在着一定的机制使得细胞摄取GEM降低。Objective To investigate how to induce the resistant cell line SW1990/gemcitabine（ GEM） for further clarify the resistant mechanisms of pancreatic cancer , and compare the characteristics between SW1990 and SW1990-GZ. Methods SW1990-GZ was derived from the human pancreatic cancer cell lines SW1990 by exposing the cells to intermittently increasing concentrations of GEM. The IC50 ,resistance index（ R） , and growth difference was observed by MTT assay. Cellular intake of SW1990 and SW1990-GZ was detection by HPLC. Results The IC50 of SW1990 and SW1990-GZ were respectively （0. 07 ± 0. 002 1）μg/ml,（87. 50 ± 3. 240 0）μg/ml,R=1 250;sensibility to GEM in SW1990-GZ were more lower than that in SW1990 cell in different concentration s of GEM. Growth rate of SW1990-GZ was slower than that of SW1990 from the growth curve;GEM intake in SW1990-GZ was lower than that in SW1990 in different times. Conclusion Human resistant pancreatic cancer cell lines SW1990/GEM is successfully established,and its resistant characteristics are stable and clear. SW1990-GZ maybe decrease GEM intake by some unclear pathways.

关 键 词：人类胰腺癌细胞株 吉西他滨耐药 建立 

分 类 号：R735.9[医药卫生—肿瘤]