三七三醇皂苷对糖尿病大鼠学习记忆能力及星形胶质细胞活性的影响  

Roles of panaxtrial saponins in cognition and memory of diabetic rat and in activity of astrocytes

作　　者：李春桃[1] 韩言秀 杜晓薇[2] 左中夫[1] 

机构地区：[1]辽宁医学院解剖学教研室,121001 [2]辽宁医学院基础医学院

出　　处：《天津医药》2015年第4期364-366,I0003,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81300931);辽宁省自然科学基金资助项目(2013022055);辽宁省大学生创新创业训练计划项目(201310160004);辽宁医学院"校长基金"资助项目(XZJJ20130105-03);2013年度辽宁医学院大学生科技创新项目

摘　　要：目的探讨三七三醇皂苷(PTS)对糖尿病大鼠学习记忆的改善作用,并从星形胶质细胞角度揭示其机制。方法 24只SD大鼠随机分为对照组、糖尿病组及PTS治疗组,每组8只。于SD大鼠腹腔注射链脲佐菌素诱导糖尿病模型,对照组为正常SD大鼠,PTS治疗组为糖尿病大鼠给予PTS治疗。检测大鼠血糖和体质量,3个月后,水迷宫实验观察各组大鼠的学习记忆功能,免疫组化染色检测脑海马区星形胶质细胞形态,Western blot法检测神经胶质纤维酸性蛋白(GFAP)及胶质细胞源性神经营养因子(GDNF)表达。结果糖尿病组较对照组学习记忆能力减退,脑海马区星形胶质细胞胞体萎缩、突起变细,脑海马区GFAP及GDNF表达明显减少(均P<0.05)。PTS组较糖尿病组学习记忆能力改善,脑海马区星形胶质细胞形态改善、GFAP及GDNF表达明显恢复(均P<0.05)。结论 PTS能恢复糖尿病大鼠脑海马区星形胶质细胞活性,恢复GDNF表达,提高糖尿病大鼠学习记忆功能。Objective To explore protective effects of panaxtrial saponins （PTS） on cognition and memory of diabetic rats and to reveal its mechanism by which might involve regulating activity of astrocytes. Methods SD rats （n=24） were ran？domly assigned into control, diabetic and PTS-treated groups （n=8 in each group）. Rat diabetic model was induced through streptozotocin injection intraperitoneally. Rats in control group were native rats, and rats in PTS-treated group were diabetic rats that were administered with PTS. Body weight and blood glucose were monitored through the experiments. Three months later, state of cognition was examined by methods of water maze. Hippocampal astrocyte morphology were detected by immu？nohistochemistry, and the expression of glial cell line-derived neurotrophic factor （GDNF） and glial fibrillary acidic protein （GFAP） in hippocampus were revealed by Western blot. Results Compared with control group, diabetic group showed cog？nitive dysfunction, atrophic astrocyte soma, shrinked astrocyte processes, and down-regulation of hippocampal GFAP and GDNF （P〈0.05）. Compared with diabetic group, PTS-treated group exhibited improved cognition and morphology of hippo？campal astrocyte, and reversed expression of GFAP and GDNF in diabetic hippocampus （P〈0.05）. Conclusion PTS re？versed astrocytic reactivity as well as expression of GDNF and GFAP in diabetic hippocampus and ameliorated diabetic cog？nitive dysfunction.

关 键 词：糖尿病 神经胶质原纤维酸性蛋白质 模型 动物 学习记忆 三七三醇皂苷 星形胶质细胞 胶质细胞源性神经营养因子 

分 类 号：R742[医药卫生—神经病学与精神病学]