膜-细胞骨架结合磷酸化蛋白50基因与乳腺癌特异性基因1在子宫内膜癌中表达及意义  

Expressions and significances of ezrin-radixin-moesin-binding phosphoprotein 50and breast cancer-specific gene 1in endometrial cancer

作　　者：赵林[1,2] 

机构地区：[1]川北医学院附属第二医院 [2]绵阳市四零四医院妇产科,四川绵阳621000

出　　处：《中华实用诊断与治疗杂志》2015年第4期352-354,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：四川省卫生厅基金资助项目(130438)

摘　　要：目的探讨膜-细胞骨架结合磷酸化蛋白50基因(ezrin-radixin-moesin-binding phosphoprotein 50,EBP50)与乳腺癌特异性基因1(breat cancer-specific gene 1,BCSG1)在子宫内膜癌中的表达及相互关系。方法子宫内膜癌患者67例为子宫内膜癌组,子宫内膜非典型增生患者68例为增生组,子宫内膜正常患者70例为对照组,分别取3组患者的子宫内膜癌组织、非典型增生组织和子宫内膜正常组织,采用免疫组织化学法检测3组EBP50及BCSG1蛋白表达水平,采用荧光定量PCR检测EBP50及BCSG1mRAN表达水平。结果子宫内膜癌组EBP50和BCSG1mRNA表达水平(2.58±1.20、0.84±0.10)以及其蛋白阳性率(88.05%、79.10%)均高于增生组(1.76±0.53、0.58±0.14和35.29%、27.94%)及对照组(1.06±0.65、0.21±0.20和12.85%、5.71%)(P<0.05),增生组高于对照组(P<0.05);子宫内膜癌组EBP50mRAN表达与BCSG1mRAN表达呈正相关(r=0.725,P=0.001)。结论 EBP50和BCSG1基因均与子宫内膜癌的发生、发展有关,EBP50和BCSG1对子宫内膜癌的发生和调控有协同作用。Objective To explore the expressions of ezrin-radixin-moesin-binding phosphoprotein 50（EBP50）and breast cancer-specific gene 1（BCSG1）in endometrial cancer and their relationship.Methods The EBP50 and BCSG1proteins and mRANs were detected by immunohistochemical technique and fluorescence quantitative PCR in 67 patients with endometrial cancer（cancer group）,68 patients with atypical hyperplasia（hyperplasia group）and 70 patients with normal endometrial tissue（control group）.Results The levels of EBP50 and BCSG1mRNAs,and the positive rates of EBP50 and BCSG1proteins were higher in cancer group（2.58±1.20,0.84±0.10;88.05%,79.10%）than those in hyperplasia group（1.76±0.53,0.58±0.14;35.29%,27.94%）and control group（1.06±0.65,0.21±0.20;12.85%,5.71%）（P〈0.05）,and higher in hyperplasia group than those in control group（P〈0.05）.EBP50 mRAN was positively correlated with BCSG1 mRAN in cancer group（r=0.725,P=0.001）.Conclusion EBP50 and BCSG1genes are involved in the development and progression of endometrial cancer.They have synergic effect on the development and regulation of endometrial cancer.

关 键 词：子宫内膜癌 EBP50 BCSG1 免疫组织化学 PCR 

分 类 号：R737.33[医药卫生—肿瘤]