稳定转染小鼠IL-17全长基因的结肠癌细胞株的建立及基因转染对C26细胞的影响  

Construction of a transfected colon cancer cell line expressing mouse IL-17 and the effect of gene transfection on C26 cell

作　　者：李艳双[1,2] 姚智燕[2] 张建平[3] 杨丽娟[2] 

机构地区：[1]河北联合大学临床医学院医学转化医学实验室,唐山063000 [2]河北医科大学基础医学院免疫教研室河北省重大疾病的免疫机制及干预重点实验室,石家庄050017 [3]石家庄市第一医院老年病三科,050017

出　　处：《免疫学杂志》2015年第4期366-368,共3页Immunological Journal

基　　金：河北省教育厅基础研究项目(2011101)

摘　　要：目的建立稳定转染小鼠IL-17基因全长的小鼠结肠癌C26细胞株并进行鉴定。方法脂质体法将携带小鼠IL-17基因全长的真核表达载体pc DNA3.1转染小鼠结肠癌细胞C26,经G418筛选出稳定表达IL-17的细胞株,镜下观察细胞形态,RTPCR法、免疫荧光法检测目的基因和蛋白的表达;划痕修复实验检测细胞的迁移能力,MTS法检测细胞体外增殖能力。结果获得1株稳定表达IL-17的C26细胞,C26/IL-17细胞高表达IL-17基因及蛋白,证明该细胞可表达IL-17;IL-17高表达可以增强C26细胞的迁移能力,减弱该细胞的体外增殖能力。结论成功建立了稳定转染IL-17基因的小鼠结肠癌细胞株。This study designed to establish a stable mouse full-length IL-17 gene-transfected C26 cell line and identify its function. C26 mouse colon cancer cell line was transfected with IL-17 full length gene（inserted into pc DNA-3.1 vector） using lipofectamine 2000 and selected with G418. Then cellular morphology, target gene, and protein expressions of the cells were analyzed by RT-PCR and immunofluorescence; the migration and proliferation of the cells in vitro were detected. Data showed C26 cell line stably transfected with mice full length IL-17 was constructed. In conclusion, IL-17 gene could highly express in C26/IL-17 cell, suggesting that this cell line could express functional IL-17. So we have successfully established a mouse colon cancer cell line which is stably transfected with IL-17 gene.

关 键 词：白细胞介素17 转染 结肠肿瘤 

分 类 号：R735.35[医药卫生—肿瘤]