抑制细胞间缝隙连接通讯功能对星形胶质细胞缺氧/复氧损伤的保护作用  

Inhibition of gap junctional intercellular communication protects astrocytes from hypoxia/reoxygenation injury

作　　者：童旭辉[1] 谷昱琛[1] 焦浩[1] 于丽[1] 董淑英[1] 

机构地区：[1]蚌埠医学院药学系药理学教研室

出　　处：《浙江大学学报（医学版）》2015年第1期67-73,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(81402930);安徽省自然科学基金(1408085MH176)

摘　　要：目的：研究抑制细胞缝隙连接通讯功能对星形胶质细胞缺氧／复氧损伤的影响。方法：原代培养的新生SD大鼠大脑皮层星形胶质细胞分为正常对照组、空白对照组及细胞缝隙连接抑制剂18－α－甘草次酸和油酸酰胺组。荧光示踪法检测星形胶质细胞的缝隙连接通讯功能；MTT法检测抑制缝隙连接通讯功能对缺氧／复氧损伤星形胶质细胞存活率的影响；Annexin V／碘化丙啶双染流式细胞术及Hoechst 33258荧光染色法检测抑制缝隙连接通讯功能对缺氧／复氧损伤星形胶质细胞凋亡的影响。结果：与正常对照组相比，空白对照组星形胶质细胞缝隙连接通讯功能明显增强（P＜0．01），细胞存活率明显降低（P＜0．01），细胞凋亡明显增加（ P＜0．01）；与空白对照组相比，18－α－甘草次酸及油酸酰胺可显著降低星形胶质细胞缝隙连接通讯功能（均P＜0．01） ，减轻缺氧／复氧损伤引起的星形胶质细胞存活率的降低（均P＜0．01） ，且可减少缺氧／复氧损伤引起的星形胶质细胞凋亡（均P＜0．01）。结论：抑制细胞间缝隙连接通讯功能对星形胶质细胞缺氧／复氧损伤具有保护作用。Objective： To investigate the effects of inhibiting gap junctional intercellular communication on hypoxia/reoxygenation injury in astrocytes .Methods：Primary cultured cerebral cortical astrocytes of neonate rats were divided into normal control group , hypoxia reoxygenation injury group and 18-α-glycyrrhetinic acid and＆amp;nbsp;oleamide （ gap junctional intercellular channel inhibitors ） group.The gap junction intercellular communication was determined by Parachute assay . The viability of astrocyes was detected by MTT assay .The apoptosis of astrocytes were detected with annexin V/PI and Hoechst 33258 staining .Results：Compared with the normal control group , the gap junctional function of astrocytes was increased significantly in ischemia /reperfusion group （ P 〈0.01 ） , the surviving fraction of astrocytes decreased significantly （ P〈0 .01 ） and its cell apoptosis ratio increased significantly （ P〈0 .01 ） . Compared with the ischemia/reperfusion group , the gap junctional function of astrocytes in18-α-glycyrrhetinic acid and oleamide group decreased significantly （ P〈0 .01 ） , the viability of astrocytes increased significantly （P〈0.01）, while cell apoptosis decreased significantly （ P 〈0 .01 ） .Conclusion： Inhibition of intercellular gap junction has protective effect against hypoxia/reoxygenation injury in astrocytes .

关 键 词：缝隙接合部/生理学 缺氧 星形细胞/病理学 甘草次酸/药理学 酰胺类/药理学 油酸 细胞凋亡 

分 类 号：R741[医药卫生—神经病学与精神病学]